Safe and effective method for chronic 17beta-estradiol administration to mice.

Supraphysiological 17beta-estradiol treatment administered via subcutaneous pellets is commonly used in mice. However, despite its efficacy in eliciting a uterotrophic response, we demonstrate that this regimen also was associated with urine retention, hydronephrosis, and ultimately premature death. To determine a safer yet still effective method to chronically treat mice with 17beta-estradiol, we initiated a placebo-controlled study to treat ovariectomized C57BL/6J mice for 6 weeks with various doses of 17beta-estradiol administered either in their drinking water (0.

Evaluation of an antimicrobial-impregnated continuous ambulatory peritoneal dialysis catheter for infection control in rats.

Infection is the most serious complication arising in long-term continuous ambulatory peritoneal dialysis (CAPD), specifically peritonitis and exit-site infection. The initial weeks after implantation is the crucial period during which bacterial colonization of the catheter results in maximal morbidity, with Staphylococcus aureus being the most virulent organism. We developed an antimicrobial-impregnated CAPD catheter by impregnating the cuff and tubing with chlorhexidine, silver sulfadiazine, and triclosan in a polymer matrix.