Challenges and opportunities in newly diagnosed glioblastoma in the United Kingdom: A Delphi panel.

Background: Glioblastoma is the deadliest primary malignant brain tumor in adults with limited treatment options and an average survival time of 12-18 months in the United Kingdom. In addition, glioblastoma has a highly detrimental impact on physical, cognitive, and emotional well-being, leaving substantial unmet needs for patients and caregivers. This study aimed to identify unmet needs in people with newly diagnosed glioblastoma and opportunities to mitigate them.

Extracellular acidification increases uterine contraction in pregnant mouse by increasing intracellular calcium.

Aims: As uterine extracellular pH decreases during the ischemic conditions of labor, but its effects on myometrial contraction are largely unknown, there is a need to elucidate its physiological effects and mechanisms of action. Furthermore, it is not known if any of the effects of extracellular acidification are affected by pregnancy, thus we also determined how gestation affects the response to acidification.

Methods: Nonpregnant, mid-, and term-pregnant myometrial strips were obtained from humanely killed mice.

Lecture Capture: Friend or Foe?

Lecture capture (LC)-a recording of the live lecture provided as a supplementary resource-is accepted as a standard provision in UK higher education. Previous research has shown it to be very popular with students, although there have been conflicting findings in terms of its impact on attendance and attainment, and suggestions that student engagement with this resource varies depending on their own preferences and approaches. The aim of the present study was to determine the impact of LC on students in a wider sense, encompassing pedagogic and pastoral aspects of student development.

Peer-assisted learning - an antidote for spoonfeeding? Reflections on peer-assisted learning activites in a veterinary curriculum.

This article was migrated. The article was marked as recommended. Peer-assisted learning (PAL) is a potentially valuable teaching tool for students on veterinary and medical curricula, helping them to develop crucial learning, teaching and meta-adaptive skills ( Lizzio & Wilson, 2004) which will serve them during their undergraduate studies and throughout their future careers.

Inhibitory effect of visfatin and leptin on human and rat myometrial contractility.

Aims: The purpose of this study was to investigate the effect of visfatin on in vitro myometrial contractility in human and rat, and compare it to leptin.

Main Methods: Myometrial strips from term pregnant women having a caesarean section or rats were dissected, superfused with physiological saline and the effects of visfatin (500pM-25nM) or leptin (1nM-1μM), on spontaneous and oxytocin-induced contractions were studied. After establishment of regular contractions, tissues were incubated for control and test response at 37°C for 20min, and then contractility was assayed.

Myometrial physiology--time to translate?

In this short review, we discuss how recent insights into myometrial physiology may be taken forward and translated into much-needed novel therapies for problems associated with labour. We consider excitation-contraction coupling in the myometrium and how this relates to our understanding of the changes that occur to produce myometrial contractions and successful labour. We then discuss how this information has already been used in the development of drugs to either stimulate or relax the myometrium, to address the needs of women with either slow (dystocic) labours or threatened preterm labours, respectively.

What do we know about what happens to myometrial function as women age?

Much has been written about the effects of aging on reproductive function, especially female fertility. Much less is known about how aging may affect the contractility of the smooth muscle within the uterus, the myometrium. The myometrium is active through a woman's entire life, not just during pregnancy.

Distribution, expression and functional effects of small conductance Ca-activated potassium (SK) channels in rat myometrium.

Calcium-activated potassium channels are important in a variety of smooth muscles, contributing to excitability and contractility. In the myometrium previous work has focussed on the large conductance channels (BK), and the role of small conductance channels (SK) has received scant attention, despite the finding that over-expression of an SK channel isoform (SK3) results in uterine dysfunction and delayed parturition. This study therefore characterises the expression of the three SK channel isoforms (SK1-3) in rat myometrium throughout pregnancy and investigates their effect on cytosolic [Ca] and force and compares this with that of BK channels.

A review of recent insights into the role of the sarcoplasmic reticulum and Ca entry in uterine smooth muscle.

The uterine sacroplasmic reticulum (SR) takes up and stores calcium [Ca], using an ATPase (SERCA) and the Ca-buffering proteins, calsequestrin and calreticulin. This stored Ca can be released via IP(3)-gated Ca channels. Decreases in luminal Ca concentration [Ca] have been directly measured following agonist stimulation.

In situ calcium signaling: no calcium sparks detected in rat myometrium.

Controlled uterine smooth muscle activity is essential for our reproductive health. While we understand reasonably well the steps that produce contraction following a rise in intracellular [Ca], the mechanism controlling excitability and thus the rise of Ca, is less well understood. Here we examine the role of the internal Ca sore, the sarcoplasmic reticulum (SR), and its relation to surface membrane ion channels.

Lipid rafts, the sarcoplasmic reticulum and uterine calcium signalling: an integrated approach.

The pathways involved in Ca2+ signalling in the uterus remain incompletely understood, impairing our ability to prevent preterm and difficult labours. In this review we focus on two elements in the pathway of Ca2+ signalling that have recently emerged as playing important roles: membrane lipid rafts and the sarcoplasmic reticulum. We examine the evidence for lipid rafts in the uterus and discuss their functional role.

Calcium signalling in smooth muscle.

Calcium signalling in smooth muscles is complex, but our understanding of it has increased markedly in recent years. Thus, progress has been made in relating global Ca2+ signals to changes in force in smooth muscles and understanding the biochemical and molecular mechanisms involved in Ca2+ sensitization, i.e.

The role of the sarcoplasmic reticulum in neonatal uterine smooth muscle: enhanced role compared to adult rat.

Little is known about contractile activity, response to agonists or excitation-contraction coupling in neonatal smooth muscle. We have therefore investigated 10-day rat uterus to better understand these processes, and compared it to adult uterus to elucidate how control of contractility develops. Spontaneous contractions are present in the 10-day neonatal uterus, although they are not as large or as regular as those present in adult tissues.