Genome sequence and analysis of a Propionibacterium acnes bacteriophage.

Cutaneous propionibacteria are important commensals of human skin and are implicated in a wide range of opportunistic infections. Propionibacterium acnes is also associated with inflammatory acne vulgaris. Bacteriophage PA6 is the first phage of P.

Characterisation of cryptic plasmid pPG01 from Propionibacterium granulosum, the first plasmid to be isolated from a member of the cutaneous propionibacteria.

A cryptic plasmid, pPG01 (3539bp), was isolated from Propionibacterium granulosum and sequenced. Analysis of open reading frames (ORFs) predicted pPG01 to encode three proteins. The largest protein (447 amino acids) showed homology to the FtsK/SpoIIIE family of proteins involved in chromosome partitioning during cell division and conjugal transfer of DNA.

Regulation of postangiogenic neovessel survival by beta1 and beta3 integrins in collagen and fibrin matrices.

We used the aortic ring model of angiogenesis to investigate the role of beta(1) and beta(3) integrins in postangiogenic vascular survival in collagen and fibrin matrices. Confocal microscopy studies showed that both beta(1) and beta(3) integrins were expressed in endothelial cells and pericytes of sprouting neovessels. Antibody blocking experiments demonstrated that beta(1) integrins but not beta(3) integrins were required for angiogenic sprouting in collagen.

A new ex vivo model to study venous angiogenesis and arterio-venous anastomosis formation.

Explants of rat inferior vena cava embedded in collagen gel and cultured under serum-free conditions produced microvascular outgrowths composed of endothelial cells and pericytes. Exogenous vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) dose-dependently stimulated angiogenesis and induced the formation of complex networks of highly branched microvessels. VEGF and the VEGF/bFGF combination also promoted pericyte recruitment.