Suppression by L-methionine of cell cycle progression in LNCaP and MCF-7 cells but not benign cells.

Background/aim: Methionine inhibits proliferation of breast and prostate cancer cells. This study aimed to determine cell cycle effects of methionine and selectivity for cancer cells.

Materials And Methods: MCF-7 (breast), LNCaP (prostate), and LS-174 (colon) cancer cells (wild-type p53), DU-145 (prostate) and SW480 (colon) cancer cells (mutated p53), and immortalized, non-tumorigenic MCF-10A (breast), BPH-1 (prostate), and NCM-460 (colon) epithelial cells were used.

Evidence of increased DNA content of murine thymocytes caused by cocaine.

C57BL mice were injected intraperitoneally daily with 10 to 50 mg/kg of cocaine for five days. Four hours after the last injection, the thymuses were removed, and the effects on DNA and protein content of the surviving cells were measured. A dose-dependent increase in DNA content per cell, determined by UV spectrophotometric analysis, and a dose-dependent increase in protein content per cell, as measured using the Bio-Rad reagent, were observed.