A Non-Volatile Pro-Dicamba Herbicide Inspired by Meclofenoxate.

Dicamba is a popular herbicide with rising use but is also notorious for volatility drift. Inspired by meclofenoxate, which we show to be highly herbicidal, we developed a derivative of dicamba with an ester-bond to 2-dimethylaminoethanol. It remained herbicidal but is non-volatile, entering plants intact and hydrolyzing inside leaves to dicamba and 2-dimethylaminoethanol.

Bioinformatic, Enzymatic, and Structural Characterization of Hexosaminidase HEX-2.

Hexosaminidases are key enzymes in glycoconjugate metabolism and occur in all kingdoms of life. Here, we have investigated the phylogeny of the GH20 glycosyl hydrolase family in nematodes and identified a β-hexosaminidase subclade present only in the Dorylaimia. We have expressed one of these, HEX-2 from , a porcine parasite, and shown that it prefers an aryl β--acetylgalactosaminide .

A fungal tolerance trait and selective inhibitors proffer HMG-CoA reductase as a herbicide mode-of-action.

Decades of intense herbicide use has led to resistance in weeds. Without innovative weed management practices and new herbicidal modes of action, the unabated rise of herbicide resistance will undoubtedly place further stress upon food security. HMGR (3-hydroxy-3-methylglutaryl-coenzyme A reductase) is the rate limiting enzyme of the eukaryotic mevalonate pathway successfully targeted by statins to treat hypercholesterolemia in humans.

Crystal structure of Arabidopsis thaliana HPPK/DHPS, a bifunctional enzyme and target of the herbicide asulam.

Herbicides are vital for modern agriculture, but their utility is threatened by genetic or metabolic resistance in weeds, as well as regulatory barriers. Of the known herbicide modes of action, 7,8-dihydropterin synthase (DHPS), which is involved in folate biosynthesis, is targeted by just one commercial herbicide, asulam. A mimic of the substrate para-aminobenzoic acid, asulam is chemically similar to sulfonamide antibiotics, and although it is still in widespread use, asulam has faced regulatory scrutiny.

Inhibition of chloroplast translation as a new target for herbicides.

The rise in herbicide resistance over recent decades threatens global agriculture and food security and so discovery of new modes of action is increasingly important. Here we reveal linezolid, an oxazolidinone antibiotic that inhibits microbial translation, is also herbicidal. To validate the herbicidal mode of action of linezolid we confirmed its micromolar inhibition is specific to chloroplast translation and did not affect photosynthesis directly.