Immersive learning laboratory in health and nursing: learning biosafety in a virtual world.

Objectives: to develop a virtual simulation and learning laboratory in an immersive virtual world that enables students, nursing professionals as well as health professionals to experiment, reflect and contextualize biosafety actions, infection protection concepts and universal precautions.

Methods: description of the development of the Immersive Learning Laboratory in Health and Nursing (LIASE) with a focus on biosafety, having as theoretical basis Kolb's experimental learning cycle and the OpenSimulator platform.

Results: development of the Immersive Learning Laboratory in Health and Nursing: Health Biosafety Module - contains five learning stations.

Antioxidant activity of and -amyrin isolated from leaves.

Brazil has a great variety of native plants which could be explored and among them guabiju () stands out. Thus, this study consisted of isolating pentacyclic triterpenes, and -amyrin from guajibu leaves, and determine the antioxidant activity.The leaves were dried, pulverized and submitted to a dynamic maceration process with ethanol 96° GL, concentrated to obtain crude leaf extract (CLE).

Rickettsia amblyommatis infecting Amblyomma pseudoconcolor in area of new focus of spotted fever in northeast Brazil.

In Brazil, active infections of Rickettsia spp. is confirmed in all regions, involving various species of ticks. During investigation of a new focus of Spotted Fever (SF) incidence in the state of Pernambuco, northeastern Brazil, tick species Rhipicephalus sanguineus sensu lato (Latreille), and Amblyomma pseudoconcolor were collected from one Canis familiaris and four Euphractus sexcinctus, respectively, and analized for the presence of rickettsial genes.

Spotted Fever: Epidemiology and Vector-Host Relationship in Rio de Janeiro State.

The eco-epidemiological scenario of spotted fever (SF), a tick-borne disease that affects humans and other animals in several countries around the world, was analyzed in Rio de Janeiro (RJ) State, Brazil. During the last 34 years, 990 SF cases were reported in RJ (the Brazilian state with the highest population density), including 116 cases confirmed by serology (RIFI) or PCR, among 42.39% of the municipalities with reported cases of SF.

DnaK and GroEL are induced in response to antibiotic and heat shock in Acinetobacter baumannii.

We studied the expression of DnaK and GroEL in Acinetobacter baumannii cells (strains ATCC 19606 and RS4) under stress caused by heat shock or antibiotics. A Western blot assay showed that DnaK and GroEL levels increased transiently more than 2-fold after exposure of bacterial cells to heat shock for 20 min at 50 degrees C. Heat induction of DnaK and GroEL was blocked completely when an inhibitor of transcription, rifampicin, was added 1 min before a temperature upshift to 50 degrees C, suggesting that the induction of these chaperones depends on transcription.