Publications by authors named "Karel Thooft"
Article Synopsis
- VHHs are unique antigen binders with potential for therapies, research, and diagnostics due to their small size and stability.
- A structure-guided method was used to identify specific regions in VHHs where N-glycosylation can occur without disrupting protein function or ability to bind to antigens.
- The study revealed that glyco-engineered VHHs can efficiently target macrophages in the lungs, highlighting a promising application for selective drug delivery using these modified proteins.
Org Biomol Chem
January 2022
Article Synopsis
- A new protein conjugation method called GlyConnect-oxime (GC) has been developed as an extension to the existing GlycoDelete (GD) technology, which allows for site-selective attachment of glycans to proteins.
- The GlycoDelete technique creates glycoproteins with specific types of glycans, making it easier to perform site-selective conjugation due to their unique properties.
- The current research explores using multicomponent reactions, like Ugi and Passerini, to enhance this process by allowing multiple groups to be introduced in one step while maintaining a hydrophilic peptide-like linker.
