Expanding Direct Coupling Analysis to Identify Heterodimeric Interfaces from Limited Protein Sequence Data.

Direct coupling analysis (DCA) is a global statistical approach that uses information encoded in protein sequence data to predict spatial contacts in a three-dimensional structure of a folded protein. DCA has been widely used to predict the monomeric fold at amino acid resolution and to identify biologically relevant interaction sites within a folded protein. Going beyond single proteins, DCA has also been used to identify spatial contacts that stabilize the interaction in protein complex formation.