Identification of HPV DNA in patients with juvenile-onset recurrent respiratory papillomatosis using SYBR Green real-time PCR.

Objective: Recurrent respiratory papillomatosis (RRP) is the most common benign neoplasm affecting the larynx and upper respiratory tract in children. Human papillomavirus (HPV) has been implicated as the cause of RRP, most commonly types 6 and 11. The present study was undertaken to evaluate the occurrence of HPV types in a group of patients with juvenile-onset RRP (JORRP).

Significance of M-cells in the panautoprotection of the body.

Life consists of continuous acceptance, adequate processing and appropriate use of energy-carriers present in and around the body, along with effective elimination of those of them which invade the organism from outside, or appear inside, thus threatening its existence. It is clear that all cells making up the organism are naturally involved in its protection. Since no "extracorporal bodyguards" have been detected so far, recently I introduced the concept of panautoprotection in the human organism, which was subsequently expanded to comprise perhaps the broadest and most sufficiently exhausting paradigm on the essence of life and other known events in the Universe.

Surfactant-producing epithelium in the dorsal part of the cartilaginous eustachian tube of mice. Light, transmission, and scanning electron microscopic observations.

The nonciliated area in cartilaginous roof tubothelium of 14 conventional mice was examined histologically and classified as a modified transitional respiratory epithelium. On the free cell surface numerous short microvilli were found. On the lower roof surface in the midcartilaginous portion and especially near the pharyngeal orifice, a convoluted pattern of ridges and pits was observed.

Lipoid proteinosis.

Lipoid proteinosis (Urbach-Wiethe disease) is a rare, recessively inherited disorder that is characterized by the deposition of hyaline-like material in the skin, oral cavity, and other tissues. It usually appears in infancy with hoarseness. We report a case of lipoid proteinosis in a 10-year-old boy that demonstrates the characteristic clinical, histologic, and ultramicroscopic features of this disease.

[Ultrastructural studies of Klebsiella rhinoscleromatis].

In 1870, F. Hebra described a slowly progressing granulomatous disease of the nasal mucous membrane he called rhinoscleroma. Later, N.

Amyloid-like protein in children with rhinoscleroma.

Mucosal specimens from two children with rhinoscleroma have been investigated by light-, fluorescent- and electron microscopy. Amyloid-like protein was found, located not only in the vessel walls but also impregnated in the basic substance of the connective tissue. In this paper the genesis of this protein is discussed, assuming that it could be a result of auto-immune reactions.

Specialization of tonsils as analyzers of the human immune system.

On the basis of current knowledge on the structure and function of tonsils, in agreement with the author's concept on "panautoprotection" in the human body, an attempt at characterizing tonsils as analyzers of the immune system is made. It is suggested that adequate signals for these analyzers are high-molecular substances penetrating orally and nasally. It is emphasized that tonsillar specialization as immunological analyzers refers mainly to the structure of their surface epithelium, the latter being considered as a peripheral component of these analyzers.

Phagocytic activity and bactericidal capacity of polymorphonuclear leukocytes in children with recurrent otitis media.

In 19 children with recurrent otitis media the phagocytic activity of blood granulocytes was studied and indirectly also their bactericidal capacity by means of the NBT test and myeloperoxidase activity. The mean value of granulocytes containing latex particles was 44.32% in the patients group, which differed significantly from the mean value established in controls: 66.

M-cells in the epithelium of the nasopharyngeal tonsil.

Electron-microscope investigation on the epithelium was carried out in 23 nasopharyngeal tonsils. Adenoidectomy was performed on the ground of recurrent adenoiditis in the interattacks period in 13 boys and 10 girls between the ages of 2 to 4. The presence of five characteristic cellular types was found on the surface of the adenoid epithelium: tall cylindrical ciliated cells; cylindrical cells covered only with microvilli; secretory cells with the predominance of the so-called "dark granules"-producing cells; flat epithelial cells; cubic cells with microvilli on the surface and many vesicles in the cytoplasm.

Electron-microscope observations on the tonsillar epithelium in children with recurrent tonsillitis.

Twenty tonsils from children aged 3-6 years removed by operation due to recurrent tonsillitis have been examined. Electron-microscope observations of the epithelial layer have shown a lowering of the number and a reduction of the tubulo-vesicular system of the M-cells. The squamous stratified epithelium is predominating; it is keratinized in some places.