[Problems of expert assessment of a case of fatal rupture of the wall of the rudimentary horn of the uterus in ectopic pregnancy].

Recently in the Russian Federation there has been a significant increase in the number of commissions of forensic medical examinations on «medical cases» associated with inappropriate provision of medical care to the population. The problem of investigating such crimes is one of the most difficult tasks facing law enforcement agencies. A special place among them is occupied by examinations related to maternal mortality.

Electron transport in Tradescantia leaves acclimated to high and low light: thermoluminescence, PAM-fluorometry, and EPR studies.

Using thermoluminescence, PAM-fluorometry, and electron paramagnetic resonance (EPR) for assaying electron transport processes in chloroplasts in situ, we have compared photosynthetic characteristics in Tradescantia fluminensis leaves grown under low light (LL, 50-125 µmol photons m s) or high light (HL, 875-1000 µmol photons m s) condition. We found differences in the thermoluminescence (TL) spectra of LL- and HL-acclimated leaves. The LL and HL leaves show different proportions of the Q (~ 0 °C) and B (~ 25-30 °C) bands in their TL spectra; the ratios of the "light sums" of the Q and B bands being S/S ≈ 1/1 (LL) and S/S ≈ 1/3 (HL).

Slow induction of chlorophyll a fluorescence excited by blue and red light in Tradescantia leaves acclimated to high and low light.

Tradescantia is a good model for assaying induction events in higher plant leaves. Chlorophyll (Chl) fluorescence serves as a sensitive reporter of the functional state of photosynthetic apparatus in chloroplasts. The fluorescence time-course depends on the leaf growth conditions and actinic light quality.

[About the estimation of the possibility of carrying out of several self-inflicted fatal injuries by the victim].

Forensic medical experts are not infrequently faced in their practical work with the question of the possibility of inflicting body injuries by the victim to himself (herself). In such cases, the law-enforcement authorities are in the first place interested not in the possibility of such self-inflicted injuries but rather in the correspondence between the character of the injuries and the circumstances of the event that needs to be objectively established in the course of the forensic medical expertise. The cases of multiple severe self-inflicted injuries in which all the witnesses were one way or another involved in the accident as the participants of the fight with the victim, attract especially close attention and require careful examination.

[IMMUNOCHEMICAL ANALYSIS OF RECOMBINANT CHIMERIC POLYPEPTIDE OspC(gar+afz) OF BORRELIA GARINIIANIP B. AFZELI ISOLATES].

Aim: Comparative study of antigenic properties of recombinant proteins OsPCgar and OsPCafz and recombinant chimeric polypeptide OspCgar+afrz, that contains amino acid sequences of mature immune dominant OspC proteins of West-Siberian isolates of Borrelia garinii (OspCgar) and B. afzelii (OspCafz), and evaluation of possibility of their use as antigens during creation of test-systems for serodiagnostics of Lyme borreliosis (LB) on the territory of Western Siberia.

Materials And Methods: Recombinant chimeric polypeptide OSpCgar+af, and recombinant mature proteins OSPCgar and OspCafz, obtained by expression of the corresponding genes in Escherichia coli cells; purified by affinity chromatography in Ni-NTA-sepharose CL-6B and studied by EIA method for the ability to bind antibodies from sera of LB patients.

[Fluorescent Indices of Bean Leaves Treated with Sodium Fluoride].

It is shown that the treatment of bean leaves with NaF in concentration of 10(-2) M resulted in the alteration of fluorescent indices registered by the method of pulse fluorimetry. Fluorescent parameters F(0) and F(m) decreased, but the ratio F(v)/F(m) = (F(m) - F(0))/F(m), characterizing the maximal photochemical activity of photosystem II remained invariable. Photochemical fluorescence quenching (qP) was higher than in control during the first minutes of illumination with the actinic light, and it markedly decreased with the following illumination.

[Luminescent and physiological indices of triticale under the treatment of seeds with growth regulators].

It is shown that the before-sowing treatment of triticale seeds with growth regulators epin and zircon resulted in the increase in the F742/F686 ratio of the stationary fluorescence intensities of the plant leaves at the wavelength bands, 742 and 686 nm, corresponding to the maxima in the leaf fluorescence spectrum. Triticale under the treatment of seeds with growth regulators showed higher chlorophyll content, higher productivity and higher indices of the crop yield.

[Evaluation of significance of Borrelia garinii spirochete recombinant protein DbpB for serodiagnostics of ixodes tick-borne borreliosis].

Aim: Obtaining recombinant protein DbpB of West Siberia Borrelia garinii 20047 isolate and evaluation of its antigen activity for the possible use in serodiagnostics of ixodes tick-borne borreliosis (ITB).

Materials And Methods: Coding region of dbpB gene of novosibirsk B. garinii 20047 isolate was amplified by PCR and cloned as part of expressing pETm vector in Escherichia coli BL21 (DE3) strain cells.

[The extent of injuries as a criterion for the assessment of the fatal combined injury in children].

The objective of the present study was to develop the method for the comprehensive objective assessment of the combined injury for the purpose of forensic medical examination. The study material was comprised of the results of forensic medical autopsies of 580 victims of car accidents and falls from a height (303 children and 277 adults). The proposed method for the estimation of the extent of combined injuries is based on taking into account all the discovered injuries expressed as a percentage of their possible total number included in the arbitrary check-list of 110 signs.

[Luminescence characteristics of strawberry leaves in early stages of their injury by arachnoidal mite].

In this work, we have investigated how the luminescence characteristics and a total content of antioxidants in leaves of strawberry (Fragaria x ananassa Duch.) change after infestation of plants by spider mite (Tetranychus atlanticus McGregor). At the earlier stage of plant injury, the chlorophyll concentration remained unchanged.

[Biophysical methods of ecological monitoring. Photosynthetic characteristics of tree plants growing in Moscow city].

In this work, we studied an influence of ecological factors (a distance from the highway) on photosynthetic characteristics of leaves of four species of tree plants growing in Moscow city. Photosynthetic activity of leaves was assayed by instrumental methods of probing the functional state of photosynthetic apparatus, using electron paramagnetic resonance method for measuring the kinetics of photooxidation of P700 centers, thermoluminescence, and slow induction of chlorophyll fluorescence. It has been shown that kinetic parameters of the induction curves, as measured from the kinetics of photooxidation of P700 and slow induction of chlorophyll fluorescence in dark-adapted leaves, are sensible to variations of plant growing conditions.

[Slow fluorescence induction and productivity of barley treated with supercritical fluid extract of amaranth].

It is shown that treatment of barley plants with supercritical fluid extract of amaranth results in the increased fluorescence parameter (F(M) - F(T))/F(T) of slow fluorescence induction curve of the leaf. Barley treated with the extract showed higher productivity and higher indices of the yield.

[Age-specific peculiarities of the structure of fatal combined traumas in children].

The objective of this work was to study the structure and the totality of injuries associated with the fatal combined traumas in the children of different age based on the materials of forensic medical examination of children's corpses (115 original observations and 188 archived cases). The children died as a results of road traffic accidents or a fall from the height. It was shown that the structure of the combined trauma strongly depends on the children's age.

[Immunochemical analysis of recombinant protein FlaA of Western Siberian Borrelia garinii isolate].

Aim: Study of the ability of Western Siberian Borrelia garinii 20047 isolate recombinant protein FlaA to react with sera antibodies of ixodes tick borreliosis patients.

Materials And Methods: Recombinant antigen FlaA, sera of ixodes tick borreliosis patients, genetic engineering methods, solid phase EIA, and parametric and nonparametric statistical methods were used in the study.

Results: Recombinant form of mature flagellar protein FlaA of B.

[Age-specific peculiarities of manifestations of a craniocerebral injury in children and adolescents with a combined blunt trauma].

This study was designed to estimate the frequency of head injuries and selected manifestations of craniocerebral traumas in children and adolescents with a fatal combined blunt trauma. It is included 289 cases of death from a combined blunt trauma (101 original observations and data of 188 archival documents). The victims were categorized into 3 age groups.

[Peculiarities of combined blunt head injuries in children and adolescents].

The objective of the present study was to evaluate the frequency and peculiar features of combined head injuries in children and adolescents who suffered the fatal blunt trauma. A total of 101 corpses and 188 archive records were available for the analysis. The control data group was comprised of 227 original observations of adult corpses.

[Enzyme immunoassay-based analysis of OspC recombinant proteins from Borrelia garinii and Borrelia afzelii isolated in West Siberia].

Aim: To study the ability of OspC recombinant proteins from Borrelia garinii and Borrelia afzelii isolated in West Siberia to interact with serum antibodies from patients with tick-borne borreliosis (TBB).

Materials And Methods: Recombinant antigens OspC B. garinii and OspC B.

[Slow fluorescence induction and CO2-exchange in bean leaves treated with Reynoutria sachalinensis extract].

It has been shown that the treatment of bean seedlings with a water extract of Reynoutria sachalinensis increases visible photosynthesis and the activity of dark respiration of plants. The increase in CO2-uptake under relatively high light illumination (10000 1x) correlates with an increase in the fluorescence parameter (F(M) - F(T))/F(T).

[Isolation of the recombinant proteins OspC and the fragment FlaA (F-FLAA) from the western-Siberian Borrelia garinii NT29 isolates and the study of their immunochemical properties].

The structural proteins OspC and FlaA of the Lyme disease (LD) agent are known to be the basic antigens, which induce the humoral immune response at the initial stage of the disease. The goal of this work was to obtain the recombinant OspC and a fragment of the FlaA protein (f-FlaA) from the Western Siberian Borrelia garinii NT29 isolates and to assess the possibility of their use for the LD diagnosis. Encoding regions of the OspC and f-FlaB genes were amplified using PCR inserted in the pREB expressive vectors and cloned in the E.

[Influence of environmental and climatic factors on the incidence and course of stenosing laryngotracheitis in children with acute respiratory diseases].

A clear correlation was found between the development of stenosing laryngotracheitis (SLT) in the presence of viral infection, its severity, and the environment well-being of an area where a child resides. The level of environmental pollution and the interaction of environmental factors and weather-climatic ill-being, which vary with time and have regional features, adversely affect the course of SLT and require mandatory correction during complex therapy and seasonal antirecurrent treatment. The increase in the incidence of childhood SLT may be a biological indicator of the environmental ill-being of a region.

[Study of the cholinesterase active site using a fluorescent probe].

We studied fluorescence of 9-amino-1,2,3,4-tetrahydroacridine hydrochloride (tacrine) in the presence of serum cholinesterase. Quenching of tacrine fluorescence dependent on cholinesterase activity has been revealed. A quenching mechanism is proposed; it includes formation of a complex and charge transfer mediated by excited tacrine molecule as well as indole of tryptophan residue from the periphery of cholinesterase active site.

[Luminescent indicators in various parts of wheat leaves in ontogenesis].

A comparative study of slow fluorescence induction, fluorescence spectra, thermoluminescence, photosynthetic activity and chlorophyll content in ontogenesis of wheat seedlings (Triticum aestivum L, cv. Yubileinayd) grown in the laboratory conditions was carried out. It was shown that (FM-FT)/FT values of slow fluorescence induction increased with the age of leaf part, reached a maximum (for 2-week-old seedlings), and then decreased.

[Activity of the antioxidant enzymes of erythrocytes in herpetic keratitis].

Free-radical oxidations get activated, the activity of catalase slows down and the activity of superoxiddismutase increases in peripheral blood erythrocytes of patients with herpetic keratitis, which is indicative of a changing antioxidant protection in late stages of herpetic infection. The results reveal new chains in the evolution of ophthalmic herpes and confirm the feasibility of adding antioxidants to the complex therapy of patients with herpetic keratitis.

[Theoretical analysis of inductive effects in higher plant photosynthesis].

The proposed mathematical model of the light stages of photosynthesis in higher plants takes into account the regulatory process of excitation energy redistribution between photosystems depending on the redox state of electron carriers. Nonmonotonic changes in the intensity of pigment fluorescence upon switching on the excitation light (delayed fluorescence induction) are described. The time required for fluorescence to reach its peak intensity and the degree of fluorescence quenching depending on light intensity are analyzed.