Publications by authors named "Karanbir S Aulakh"
Saudi J Biol Sci
May 2020
Article Synopsis
- This study aims to optimize the regeneration process for a specific oilseed crop through -mediated transformation, achieving a callus induction frequency of 87% using MS medium with 4 µM BA after four weeks.
- Successful shoot organogenesis occurred with a 92% induction rate on MS medium supplemented with 4 µM BA and 1 µM NAA, while well-developed roots were generated using a different hormone combination.
- The research also involved the introduction of the DAcT gene into plants, resulting in a 100% regeneration frequency of transgenic shoots under specific medium conditions, with stable transformation efficiency estimated at around 7%, confirmed by PCR analysis.
