[Use of biologically active supplement containing omega-3 polyunsaturated fatty acids in patients with type 2 diabetes mellitus].

35 patients with diabetes mellitus type 2 complicated with hyperlipidemia, coronary heart disease were treated during 8 months with poseidonol--polyunsaturated fatty acid family omega 3 (PUFA--omega 3). It was shown that hypercoagulation and hypofibrinolysis in these patients occurred. Treatment with poceidonol increased plasminogen activator activity and decreased coagulation and reduced atherogenic index.

[Effect of diet therapy on status of hemocoagulation and fibrinolysis in type 2 diabetes patients].

The changes in parameters of blood coagulation and the fibrinolytic system in 60 patients with type 2 diabetes mellitus was studied. We conclude what the non insulin depended diabetes mellitus is associated with disturbances in hemostatic and fibrinolytic systems that could contribute to the development of diabetic vascular disease. Besides favorable influence to a clinical picture of disease universal normalizing influence of traditional diet therapy on parameters of coagulation and the fibrinolytic system.

[Effects of tycveinol on the lipid transport system and hemostasis and fibrinolysis systems in patients with ischemic heart disease].

33 patients with ischemic heart diseases, hyperlipidemia and blood hypercoagulation took tycveinol which consists of polyunsaturated fatty acids in a dose of 2.0 g twice a day for 2 months. A positive result manifested by a 14% drop in cholesterol, 29.

[Influence polyunsaturated fatty acids omega-3 on coagulation and fibrinolysis systems in patients with NIDDM].

35 patients with diabetes mellitus type 2 complicated with hyperlipidemia, coronary heart disease, were treated for 8 months with poseidonol which incorporates essential polyunsaturated fatty acids and fat-soluble vitamins. Poseidonol reduced atherogenic index from 4.2 to 3.

Coagulation and fibrinolysis in rats after surgery with monopolar electrical scalpel.

Blood coagulation, fibrinolysis in plasma and peritoneal fluid, and activity of tissue plasminogen activator in the peritoneum and uterine horns were studied in albino rats after surgery on the uterine horns with a monopolar electrical scalpel. This instrument induced severe inflammatory reaction and disturbances in the fibrinolysis and coagulation systems.

[Effect of the proteolytic substance morikrase on the status of scar tissue under in vitro conditions].

Proteolytical preparation moricrasa was isolated from hepatopancreas of Paralithodes camtshatic. Moricrasa and its fractions were used for lysis of cicatricial tissue under in vitro conditions. It was found that moricrasa in concentrations 0.

[A bioluminescent method of determining antibiotic sensitivity of microbial cells in septic blood].

A bioluminescence assay for rapid (5-7 h) determination of susceptibility to antibiotics was applied to samples of septic blood and optimized. The method comprises hemolysis of blood, reduction of osmolarity by adding concentrated nutritive media, and further incubation of the samples in the presence or absence of therapeutic doses of the antibiotic examined. Growth of bacteria is estimated by the level of bacterial ATP in the sample, which is determined by a bioluminescence assay.

[Role of local fibrinolytic activity of the uterine horns in rats in the pathogenesis of formation of postoperative adhesions].

Experimental models were used to examine the impact of corrections of the uterine horns on the activity of tissue plasminogen activator (tPA). The application of sutures was shown to suppress the activity of tPA in the uterine horns to a greater extent than other surgical techniques. With this, the use of catgut caused the highest suppression of tPA activity and increases in the frequency and density of formation of postoperative adhesions.

[Effect of docanol on the status of hemostasis and fibrinolysis systems, as well as lipid spectrum in patients with ischemic heart disease depending on the initial level of fibrinolytic activity].

Forty patients with coronary heart disease (CHD) concurrent with hyperlipidemia were divided into 2 groups according to the baseline fibrinolytic activity. The group with the depressed fibrinolytic system displayed the highest atherogenicity index and levels of cholesterol, low and very low density lipoprotein cholesterol and half levels of high density lipoproteins. After 45-day ingestion of docanol, a new Russian food additive containing docosahexanoic and eicosapentaenoic acids there were positive changes in the lipid spectrum in the both groups.

[The wound healing effect of ointment with morikrase].

An ointment containing, 1% morikrase (a complex of proteinases isolated from hepatopancreatic tissues of king crab Paralithodes camtschatica) exhibited the wound healing properties and did not irritate the skin tissues during long-term treatments of rats. Only slight hypocoagulation was observed in intact animals after external application of the ointment. At the same time, the drug caused an increase in fibrinolytic activity in blood of rats with purulent wounds due to activation of the plasminogen activator.

[Fibrinolytic and thrombolytic properties of the drug morikrase].

Fibrinolytic and thrombolytic properties of morikrase, a complex of proteinases obtained from hepatopancreatic tissues of king crab (Paralithodes camtschatica), were studied in vivo and in vitro. High fibrinolytic activity and dose-dependent activating properties were detected on standard and heated fibrin plates. The preparation maintained its properties during long-term storage at 4 degrees and 22 degrees C.

[Study of the thrombolytic and fibrinolytic properties of thiol- dependent serine proteinase (TSP) from Thermoactinomyces vulgaris in vivo].

Experiments on male albino rats showed that the thyol-dependent serine proteinase (TSP) dissolved the thrombus in the jugular vein for 2-5 hr. Intravenous injection of TSP activated the fibrinolytic system of intact animals by increasing the levels of the plasminogen activator and plasmin in the euglobulin fraction. The response of the fibrinolytic system on the intravenous injection of TSP (2mg/200 g) was different: in some rats, fibrinolysis was activated, while on others, it was inhibited.

[Fibrinolytic properties of protease complex isolated from the culture fluid of Nocardia minima].

The protease complex isolated form the Nocardia minima culture liquid was studied in vitro. The preparation had two different activities (fibrinolytic and activating), i.e.

[Interleukin-1 and fibrinolysis. In vivo studies].

Distinct decrease in fibrinolytic activity of both whole blood plasma and its euglobulin fraction, occurring due to liberation of an inhibitor of plasminogen activator into circulation, was observed within 2-4 hrs after a single intravenous administration of rabbit interleukin-I into rats at a dose of 50 micrograms/kg, while the activator activity was increased in kidney heart, spleen and lung tissues. The similar data were obtained if the dose was either increased up to 150 micrograms/kg or decreased down to 5 micrograms/kg and 0.5 micrograms/kg, but the effect observed proved to be dose-dependent.

[Effect of interleukin-1 on animals with a fibrinolytic dysfunction].

Activating effect of the interleukin-1 on the fibrinolytic system was shown in hypofibrinolysis models. This polypeptide exerted an opposite effect on the fibrinolysis which had been activated with i.v.

[Blood coagulation and fibrinolysis in ischemic heart disease. The relationship to risk factors].

The data on blood coagulation and fibrinolysis in patients with coronary heart disease are reviewed. The relationship of the coagulating and fibrinolytic systems with cardiovascular risk factors is analysed.

Depletion of fibrinolysis activators as a result of continuous stress.

The dynamics of disorders in haemostasis and fibrinolysis upon continuous stress was studied in model experiments with rats exposed to emotional-pain-stress. It was shown that the plasminogen activator (PA) is released into the blood within the first few minutes of exposure to stress. Four hours after cessation of continuous stress (6 hours) the enzyme depletion and fibrinolysis depression occurred.

[Fibrinolytic and thrombolytic properties of thiol-dependent serine proteinase from Thermoactinomyces vulgaris in vitro].

Fibrinolytic and thrombolytic properties of the subtilisin-like thiol-dependent serine proteinase were studied. At concentrations from 50 to 4000 micrograms/ml the enzyme causes lysis of fibrin plates and activates plasminogen. At concentrations above 100 micrograms/ml it shows a pronounced thrombolytic effect on the clots formed in vitro from both plasma and human and rat blood.

[Depression of fibrinolysis in long-term stress in the rat].

A study of fibrinolytic and hemostatic effects of emotional/painful stress induced by Dosiderato's technique in white Wistar rats demonstrated, 4 hours after discontinuation of 6-hour stress exposure, a thrombophilic condition that is characterized by heavily depressed fibrinolysis in combination with increased blood antiplasmin and coagulation activities. It is suggested that depressed fibrinolysis is a result of depletion of fibrinolytic elements, plasminogen activator in particular.

[Characteristics of hemostasis, fibrinolysis and lipid indicators in children and their fathers with a history of myocardial infarction at a young age].

Blood coagulation and fibrinolysis characteristics are described in children whose fathers survived myocardial infarction when fairly young. Hemostatic and fibrinolytic effects of heredity are analysed, as are lipid spectrum disorders. Children with aggravated heredity showed changes in plasma fibrinogen concentration, antithrombin III, total plasma fibrinolytic activity recalcification time.

[Importance of the qualitative composition of dietary proteins in the dietotherapy of ischemic heart disease patients].

The effects of two isocaloric antiatherosclerotic diets (containing 120 g protein) differing in the amino acid composition of protein sources introduced were studied and compared in patients with coronary heart disease. Conventional protein sources were used in the first diet, whereas in the second diet, 15% of protein (by weight) was replaced by soybean protein isolate. A study was also made of the effect of dietetic management on lipid metabolism in blood serum.