Bone Marrow Microenvironment-Induced Chemoprotection in Rearranged Pediatric AML Is Overcome by Azacitidine-Panobinostat Combination.

Advances in therapies of pediatric acute myeloid leukemia (AML) have been minimal in recent decades. Although 82% of patients will have an initial remission after intensive therapy, approximately 40% will relapse. is the most common chromosomal translocation in AML and has a poor prognosis resulting in high relapse rates and low chemotherapy efficacy.

The Short-chain Fatty Acid Propionic Acid Activates the Rcs Stress Response System Partially through Inhibition of d-Alanine Racemase.

The Enterobacterial Rcs stress response system reacts to envelope stresses through a complex two-component phosphorelay system to regulate a variety of environmental response genes, such as capsular polysaccharide and flagella biosynthesis genes. However, beyond Escherichia coli, the stresses that activate Rcs are not well-understood. In this study, we used a Rcs system-dependent luminescent transcriptional reporter to screen a library of over 240 antimicrobial compounds for those that activated the Rcs system in Serratia marcescens, a family bacterium.

Antibiotics Used in Empiric Treatment of Ocular Infections Trigger the Bacterial Rcs Stress Response System Independent of Antibiotic Susceptibility.

The Rcs phosphorelay is a bacterial stress response system that responds to envelope stresses and in turn controls several virulence-associated pathways, including capsule, flagella, and toxin biosynthesis, of numerous bacterial species. The Rcs system also affects antibiotic tolerance, biofilm formation, and horizontal gene transfer. The Rcs system of the ocular bacterial pathogen was recently demonstrated to influence ocular pathogenesis in a rabbit model of keratitis, with Rcs-defective mutants causing greater pathology and Rcs-activated strains demonstrating reduced inflammation.

mCloverBlaster: A tool to make markerless deletions and fusions using lambda red and I-SceI in Gram-negative bacterial genomes.

This study introduces mCloverBlaster as a genetic tool to create deletions and transcriptional and translational fusions in bacterial genomes using recombineering. The major advantage of this system is that it can be used to make deletions and fusions without leaving a selectable marker on the chromosome. mCloverBlaster has a kanamycin resistance cassette with an I-SceI restriction site flanked by fragments of the gene for the mClover3 fluorescent protein including direct repeats of mClover3 sequence on both sides of the kanamycin resistance gene.

Xylose-Inducible Promoter Tools for Species and Their Use in Implicating a Role for the Type II Secretion System Protein XcpQ in the Inhibition of Corneal Epithelial Wound Closure.

Tunable control of gene expression is an invaluable tool for biological experiments. In this study, we describe a new xylose-inducible promoter system and evaluate it in both and The promoter, derived from the operon, was incorporated into a broad-host-range pBBR1-based plasmid and was compared to the -derived promoter using as a reporter. Green fluorescent protein (GFP) fluorescence from the promoter was inducible in both species, but not in , which may facilitate the cloning of genes toxic to to generate plasmids.

Thermoregulation of Prodigiosin Biosynthesis by is Controlled at the Transcriptional Level and Requires HexS.

Several biotypes of the Gram-negative bacterium produce the tri-pyrole pigment and secondary metabolite prodigiosin. The biological activities of this pigment have therapeutic potential. For over half a century it has been known that biosynthesis of prodi giosin is inhibited when bacteria are grown at elevated temperatures, yet the fundamental mechanism underlying this thermoregulation has not been characterized.