The transcervical access for transcatheter aortic valve replacement.

Alternative accesses for transcatheter aortic valve replacement (TAVR) are needed in a certain number of patients, who present contraindications to the standard transfemoral access. We present the transcervical approach, which allows to access the aortic valve via supra-aortic vessels, namely, the brachiocephalic trunk, carotid artery, or subclavian artery. This approach is interesting because it avoids thoracotomy, which is necessary with some other alternative approaches for TAVR, such as the transaortic or transapical ones.

[Effect of lycopene on blood lipoproteids in women with type 2 diabetes mellitus in postmenopause].

Blind randomized placebo-control research included 39 women. The following criteria were: menopause period for more then 1 year long; diabetes mellitus; arterial hypertension; hypercholesterolemia. The patients were divided into two groups.

Preparation and Properties of Soluble Pig Skin Fractions Influencing on Human Keratinocyte Proliferation and Differentiation.

Three fractions with different molecular weights were isolated from pig skin. Fraction 1 (F1) and fraction 3 (F3) stimulated human keratinocyte proliferation in primary and regenerating cultures, but didn't affect their differentiation. Fraction 2 (F2) inhibited keratinocyte proliferation and stimulated their differentiation.

[Experimental validation of licopin-containing drug tomatol use in combined therapy of patients with diabetic retinopathy].

Effects of tomatol on metabolic changes in the blood and lacrimal fluid and ocular capillaries of rabbits with alloxane diabetes were studied. Tomatol is a drug containing licopin carotenoid characterized by a high biological activity. The intensity of lipid peroxidation was notably decreased, antioxidant activity of the blood increased, lipid metabolism parameters in the blood and some parameters of proteinase inhibitory balance in the blood and lacrimal fluid normalized in diabetic rabbits treated with tomatol in comparison with untreated rabbits.

[Experimental evaluation of the antibacterial activity of tomato pulp oil extract].

The antibacterial action of the oil extract obtained from tomato pulp has been studied with the use of museum strains Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 5638-P and Candida albicans ATCC 885-653, recommended for the determination of the antibacterial activity of medicinal preparations (State Pharmacopoeia, edition XI), as well as Enterobacter, Streptococcus, Staphylococcus, Klebsiella and Escherichia clinical strains. As revealed in this study, tomato pulp oil extract produces a wide-spectrum antibacterial effect on Gram-positive and Gram-negative microorganisms and on fungi of the genus Candida. The study has determined that this antibacterial action is linked with the presence of a complex of organic acids (succinic, citric, tartaric, etc.

The antioxidant properties of lycopene.

The antioxidant properties of the carotenoid lycopene were compared in three different model oxidative systems. In egg yolk liposomes, in the presence of 2.5 mM FeSO4 and 200 mM ascorbate, lycopene, alpha-tocopherol, and beta-carotene inhibited the accumulation of lipid peroxidation products reacting with 2-thiobarbituric acid (TBARS) in a dose-dependent mode, with the concentration of half-inhibition being 80, 30 and 130 mM, respectively.

[Radiation-protective effectiveness of lycopene].

Radioprotective action of natural carotenoid Lycopene in mice during exposure to lethal dose of 6.5 Gy has been shown. Lycopene was found to be two-fold more effective radioprotector than beta-carotene at the same conditions.

[Biologically active substances from the skin that influence the proliferation and differentiation of cultured human keratinocytes].

Three fractions of biologically active substances of human skin varying in molecular weight (F1 > 15 kD, F2 1.4-15 kD, F3 < 1.4 kD) were tested in primary and regenerating cultures of human epidermal keratinocytes.

[Reparation of hepatocyte mitochondrial membranes using phosphatidylcholine liposomes].

The influence of multibilayer phosphatidylcholine liposomes on the properties of rat hepatocyte mitochondria membranes damages caused by hepatotropic toxin--CCl4 was investigated. Alterations of the membrane structure were estimated by the decrease in phospholipid/protein ratio /by 33%/ and via changes of quantitative and qualitative composition of phospholipid components. The possibility of reparation of the hepatocytes mitochondria damaged membrane by egg yolk phosphatidylcholine liposomes is shown.

[Use of fistula extraction in the treatment of non-ramified pararectal fistulas].

A method of fistula extraction was used in treatment of 42 patients with non-ramified pararectal fistulas. The duration of hospital stay of the patients was 8 days, that of their out-patient treatment--14 days. Within a follow-up period of from 3 mos to 2 yrs, 1 patient with intra-sphincteric fistula developed a disease recurrency.

[Changes in the lipid composition of mitochondrial membranes during succinate oxidase reaction].

Content of lipids, free cholesterol, cholesterol esters, phospholipids and fatty acid composition were studied in mitochondria incubated in samples containing succinate. Induction of lipid peroxidation, production of malonic dialdehyde and alterations in membranes fluidity were not detected in the preparations of mitochondria within 3 hrs of succinate oxidation. Self-inactivation of mitochondria appears to occur due to an increased uncoupling of oxidative phosphorylation.

Cholesterol accumulation in plasma membrane and changes of membrane enzyme activity of Acholeplasma laidlawii cells during culture ageing.

The data presented in this report show that the microviscosity of the plasma membrane and the cholesterol/phospholipid molar ratio of its lipid bilayer increases during ageing of Acholeplasma laidawii cultures. At the same time the age changes of other lipid components content do not correlate with the change of membrane viscosity. It is also shown that membrane enzyme activities of mycoplasma cell decrease with age.

Ageing of procaryotes. Acholeplasma laidlawii as an object for cell ageing studies: a brief note.

It was established that A. laidlawii cell viability in the stationary phase of culture development decreases according to the Gompertz law. It was shown that the "stationary" ageing of A.

[Treatment of pararectal fistulas].

We observed 120 patients with acute and chronic paraproctitis. In 75 of them, the pararectal fistulas were revealed. The most frequent causes of a pararectal fistula recurrence were the incomplete excision of the necrotized fat, inadequate wound drainage and incorrect definition of the internal fistulous opening.

[Self-inactivation of NADH-oxidase from Mycoplasma cells during reaction].

The feasibility of self-inactivation of NADH-oxidase by plasma membranes of Acholeplasma laidlawii cells was investigated. It was demonstrated that the rate of NADH oxidation in a flow reactor upon stirring diminishes with time. This decrease of the reaction rate is not coupled with the presence in the reaction mixture of the reaction products--NAD+ and H2O2, and is irreversible.

[Effect of monooxygenase reactions catalyzed by cytochrome P-450 on the microsomal membrane].

Hydroxylation of dimethylaniline in rabbit liver microsomes is accompanied by inactivation of cytochrome P-450 and the formation of products inhibiting the catalytic activity of non-inactivated cytochrome P-450. Other enzymes and electron carriers of microsomal membrane (cytochrome b5, NADH-ferricyanide reductase, NADPH-cytochrome c and NADPH-cytochrome P-450 reductases) as well as glucose-6-phosphatase were not inactivated in the course of the monooxygenase reactions. Phospholipids and microsomal membrane proteins were also unaffected thereby.

[Dependence of membrane protein turnover in Mycoplasma cells on the age of the culture].

To understand the molecular mechanisms of damages appearing in biological membranes in the process of cellular aging, changes in the rate of catabolic processes in Mycoplasma cells have been studied. This study has revealed that the aging of Acholeplasma laidlawii culture is accompanied by a decrease in the activity of such catabolic enzymes as DNA-ase, RNA-ase, cathepsin D and beta-glucosidase. A considerable increase in the duration of the half-life of membrane proteins has been registered, which is indicative of a decrease in their turnover rate.