Hairy roots of Datura candida×D. aurea: effect of culture medium composition on growth and alkaloid biosynthesis.

A transformed root clone of Datura candida×D. aurea was established following infection with Agrobacterium rhizogenes strain A4. This clone was examined for its growth and hyoscyamine and scopolamine content under various culture conditions.

Production of diosgenin by hairy root cultures ofTrigonella foenum-graecum L.

Hairy root cultures ofTrigonella foenum-graecum L. were established withAgrobacterium rhizogenes strain A. The hairy roots produce diosgenin, an important spirostanol for the semi-synthesis of steroid hormones.

Production of valepotriates by hairy root cultures of Centranthus ruber DC.

Hairy root cultures of Centranthus ruber DC. were established by infection of sterile plantlets with Agrobacterium rhizogenes, strain R1601. The transformed roots were grown in 12 different, hormone-free liquid media, and valtrate, isovaltrate, 7-desisovaleroyl-7-acetylvaltrate, 7-homovaltrate, didrovaltrate and isovaleroxyhydroxydidrovaltrate were quantified by high performance liquid chromatography.

Flavonoids from Iochroma gesnerioides.

Two aglycones (kaempferol and quercetin) and three glycosides (isoquercitrin, kaempferol 3-sophoroside and quercetin 3-sophoroside) were isolated from Iochroma gesnerioides (Kunth) Miers (Solanaceae). Whereas the aglycones are common for the Solanaceae, their 3-sophorose derivatives are found only in three genera of this family. Their isolation and identification (mainly by 1H- and 13C-nmr) are reported here.

Tropane Alkaloids in Transformed Roots of Datura quercifolia.

Hairy root cultures of DATURA QUERCIFOLIA were established following infection with AGROBACTERIUM RHIZOGENES strain LBA 9402. Eight tropane alkaloids were identified in the hairy roots, hyoscyamine being the major constituent. The growth and the hyoscyamine content of transformed roots were investigated under various conditions.

High-yield production of valepotriates by hairy root cultures of Valeriana officnalis L. var. sambucifolia Mikan.

Hairy root cultures of Valeriana officinalis var. sambucifolia were established by infection of sterile plantlets with Agrobacterium rhizogenes strain R1601 The transformed roots were grown in 10 different, hormone-free liquid media and the isovaltrate, valtrate, didrovaltrate, isovaleroxyhydroxydidrovaltrate content was quantified by HPLC. Valepotriates were entirely retained inside the root tissues.

Flavonoids from Lycium halimifolium1.

Five flavonol glycosides and two genins have been isolated from the leaves of LYCIUM HALIMIFOLIUM Mill. (Solanaceae): quercetin 3- O-rutinoside-7- O-glucoside ( 1), kaempferol3- O-rutinoside-7- O-glucoside( 2), rutin ( 3), nicotiflorin ( 4), isoquercitrin ( 5), quercetin ( 6), and kaempferol ( 7). They were identified on the basis of acid and enzymatic hydrolysis and spectral data.