Publications by authors named "Kao-Chi Yang"
Article Synopsis
- Researchers developed a new way to monitor reactive oxygen species (ROS) and oxidative responses in fixed cell samples using a fluorescence probe called DCF.
- The study found that only acetone and paraformaldehyde as fixatives preserved the ability to accurately detect ROS levels during different cell cycle phases.
- This method allows for effective measurement of ROS production in fixed samples using standard staining techniques, contributing to a better understanding of cellular processes during the cell cycle.
The generation of reactive oxygen species (ROS) in a live-cell system is routinely measured using the oxidation-sensitive fluorescent probe dichlorofluorescein (DCF). However, it is difficult to simultaneously monitor cellular oxidative responses and ROS generation in cells, and analyses of cellular oxidative responses are typically performed after ROS generation has been evaluated. In this study, we developed a modified fixed staining method that allows the simultaneous analysis of ROS generation and oxidative responses using standard immunostaining techniques.
View Article and Find Full Text PDFImmune hepatic injury induced by Con A results primarily from IFN-γ-mediated inflammation, followed by hepatic cell death. Glycogen synthase kinase (GSK)-3, which acts proapoptotically and is proinflammatory, is also important for facilitating IFN-γ signaling. We hypothesized a pathogenic role for GSK-3 in Con A hepatic injury.
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