Novel Tetrazoles against Acanthamoeba castellanii Belonging to the T4 Genotype.

Background: Acanthamoeba castellanii is a pathogenic free-living amoeba responsible for blinding keratitis and fatal granulomatous amoebic encephalitis. However, treatments are not standardized but can involve the use of amidines, biguanides, and azoles.

Objectives: The aim of this study was to synthesize a variety of synthetic tetrazole derivatives and test their activities against A.