Synthesis of some novel pyrazolo[3,4-] pyrimidin-4(5)-one derivatives as potential antimicrobial agent.

Objectives: The aim of the present work was to synthesize a novel series of pyrazolo[3,4-]pyrimidin-4(5)-one derivatives and evaluate their antimicrobial activity.

Methods: Cyclization of an ortho-amino ester of 1-(2,4-dinitrophenyl)pyrazole with various aliphatic/aromatic nitriles under different reaction conditions such as conventional and microwave assisted synthesis, provided pyrazolo[3,4-] pyrimidin-4(5)-one derivatives. All the synthesized compounds were evaluated for their antimicrobial activity against selected bacteria and fungi by agar well diffusion method.