Seroprevalence of avian metapneumovirus (aMPV) among pullet and layer hens in Northern Thailand.

Avian metapneumovirus (aMPV) poses a significant global threat to the respiratory and reproductive systems of poultry, yet its prevalence and impact in specific regions, such as northern Thailand, remain understudied. This study is the first comprehensive assessment of aMPV seroprevalence among layer hens in the region. By the application of Enzyme-Linked Immunosorbent Assay (ELISA), our findings reveal a high overall seroprevalence of 60.

Persistence of passive immunity in calves receiving colostrum from cows vaccinated with a live attenuated lumpy skin disease vaccine and the performance of serological tests.

This study aimed to determine the persistent duration of maternal immunity against lumpy skin disease virus (LSDV) in dairy calves born from vaccinated cows using a virus neutralization test (VNT). The performance of the VNT and an in-house-ELISA test was also determined. Thirty-seven pregnant cows from 12 LSD-free dairy farms in Lamphun province, Thailand were immunized with a homologous Neethling strain-based attenuated vaccine and calved from December 2021 to April 2022.

Efficiency of NHEJ-CRISPR/Cas9 and Cre-LoxP Engineered Recombinant Turkey Herpesvirus Expressing OmpH Protein for Fowl Cholera Prevention in Ducks.

Fowl cholera is caused by the bacterium , a highly transmissible avian ailment with significant global implications, leading to substantial economic repercussions. The control of fowl cholera outbreaks primarily relies on vaccination using traditional vaccines that are still in use today despite their many limitations. In this research, we describe the development of a genetically engineered herpesvirus of turkeys (HVT) that carries the OmpH gene from integrated into UL 45/46 intergenic region using CRISPR/Cas9-NHEJ and Cre-Lox system editing.

Development of in-house ELISA for detection of antibodies against lumpy skin disease virus in cattle and assessment of its performance using a bayesian approach.

Lumpy skin disease (LSD) is a contagious disease among cattle and buffalo worldwide. Currently, an enzyme-linked immunosorbent assay (ELISA) has been recognized as an efficient diagnostic tool that is less time-consuming and easier than the viral neutralization test to measure the antibody levels. In the present study, an in-house method of indirect ELISA was developed to detect the bovine antibodies against Lumpy skin disease virus (LSDV) and its performance was assessed using field samples.

Simultaneous Protective Immune Responses of Ducks against Duck Plague and Fowl Cholera by Recombinant Duck Enteritis Virus Vector Expressing OmpH Gene.

Duck enteritis virus and are major duck pathogens that induce duck plague and fowl cholera, respectively, in ducks and other waterfowl populations, leading to high levels of morbidity and mortality. Immunization with live attenuated DEV vaccine containing outer membrane protein H (OmpH) can provide the most effective protection against these two infectious diseases in ducks. We have recently reported the construction of recombinant DEV expressing ompH gene using the CRISPR/Cas9 gene editing strategy with the goal of using it as a bivalent vaccine that can simultaneously protect against both infections.

Recombinant GP19 Protein as a Promising Vaccine Prototype Providing a Protective Immune Response in a Mouse Model.

The intracellular bacterium is the causative pathogen of canine monocytic ehrlichiosis (CME) in dogs. Despite its veterinary and medical importance, there is currently no available vaccine against this pathogen. In this study, the recombinant GP19 (rGP19) was produced and used as a recombinant vaccine prototype in a mouse model against experimental infection.

Incidence of hemoparasitic infections in cattle from central and northern Thailand.

Background: Hemoparasites, such as spp., spp. and spp.

Immunization of Cattle With Recombinant Structural Ectodomains I and II of Apical Membrane Antigen 1 [BbAMA-1(I/II)] Induces Strong Th1 Immune Response.

Both strong innate and adaptive immune responses are an important component of protection against intraerythrocytic protozoan parasites. Resistance to bovine babesiosis is associated with interferon (IFN)-γ mediated responses. CD4 T cells and macrophages have been identified as major effector cells mediating the clearance of pathogens.