Publications by authors named "Kannipa Tasanapak"
Background: (. ) is a common pathogen of community-acquired pneumonia. Interleukin-17 (IL-17) plays a role in host defense and contributes to disease severity in infection.
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- - This study used bioinformatics and phenotypic tests to analyze the features of a carbapenem-resistant bacteria strain, AB073, which showed resistance to seven different classes of antibiotics due to various mechanisms like altered targets and efflux.
- - AB073 could not transfer its antibiotic resistance through conjugation, and genetic analysis found no links to horizontal gene transfer, indicating that its resistance traits are likely innate rather than acquired.
- - The strain also possesses genes related to virulence factors, iron uptake, and biofilm formation, highlighting its successful spread as a pathogen in various regions and clinical settings, particularly originating from Thailand.
Article Synopsis
- * Research is focusing on new treatment methods using bacteriophages, specifically a newly isolated phage called vB_AbaAut_ChT04, which can infect many clinical strains of MDR-AB.
- * Genetic analysis of vChT04 revealed a robust genome with antimicrobial potential, leading to the development of a peptide (PLysChT04) that demonstrated effectiveness against both MDR-AB and XDR-AB in laboratory tests.*
Article Synopsis
- - The study investigates the presence of Staphylococcus aureus, a common cause of food poisoning, in restaurants in northern Thailand, focusing on its ability to produce toxins and form biofilms on food contact surfaces.
- - Researchers swabbed kitchen utensils and food handlers' hands, finding a high prevalence of contamination, particularly on hand surfaces (78%), with significant proportions of biofilm-producing strains also detected.
- - The results underscore the risk of food contamination from food handlers and utensils, highlighting the need for improved hygiene practices, like proper handwashing and cleaning of kitchen equipment, to protect consumers.
is a major cause of nosocomial infection, and the incidence of extensively drug-resistant (XDRAB) infections has dramatically increased worldwide. In this study, we aimed to explore the complete genome sequence of XDRAB 329, ST1166/98 (Oxford/Pasteur), which is an outbreak clone from a hospital in Thailand. Whole-genome sequencing (WGS) was performed using short-read Illumina and long-read PacBio sequencing, and a conjugation assay of its plasmid was performed.
View Article and Find Full Text PDFEscherichia coli, a bacterium that causes severe foodborne diseases, is transmitted to humans primarily through the consumption of contaminated foods. These foodborne pathogens are causing a public health problem that requires alternative control approaches, such as bacteriophage (phage) biocontrol. In this study, we characterized vB_EcoM_Tw01 (vTw01) isolated from sewage and vB_EcoM_Tcm05 (vTcm05) isolated from chicken meat.
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- This study explored the link between antimicrobial resistance, CRISPR/Cas systems, and virulence related to phage susceptibility in Acinetobacter baumannii strains from Thailand.
- Out of 230 strains tested, 46.5% were susceptible to phages, and this susceptibility was correlated with antibiotic resistance and biofilm formation, particularly linked to the ompA gene.
- Whole-genome analysis showed that all phage-susceptible strains carried multiple antibiotic resistance genes, biofilm-related genes, and certain CRISPR-associated genes, highlighting the relationship between bacterial virulence and their evolutionary dynamics with phages.
Conjugation is a type of horizontal gene transfer (HGT) that serves as the primary mechanism responsible for accelerating the spread of antibiotic resistance genes in Gram-negative bacteria. The present study aimed to elucidate the mechanisms underlying the conjugation-mediated gene transfer from the extensively drug-resistant Acinetobacter baumannii (XDR-AB) and New Delhi Metallo-beta-lactamase-1-producing Acinetobacter baumannii (NDM-AB) to environmental isolates of Acinetobacter spp. Conjugation experiments demonstrated that resistance to ticarcillin and kanamycin could be transferred from four donors to two sodium azide-resistant A.
View Article and Find Full Text PDFThe aspS gene encoding Aspartyl-tRNA synthetase (AspRS) from a thermotolerant acetic acid bacterium, Acetobacter pasteurianus SKU1108, has been cloned and characterized. The open reading frame (ORF) of the aspS gene consists of 1,788 bp, encoding 595 amino acid residues. The highly conserved Gly-Val-Asp-Arg ATP binding motif (motif 3) is located at the position 537-540 in the C-terminus.
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