Polysaccharide and Cyclodextrin-Based Monolithic Chiral Stationary Phases and its Application to Chiral Separation.

The recent development of monolithic chiral stationary phases (CSPs) for liquid chromatography (LC) is mainly focused on reducing backpressure, maximizing flow rates, faster run time, column efficiency, and stability. This review paper emphasizes recent progress in the development of polysaccharide and cyclodextrin-based monolithic CSPs. Further the paper draws attention to competing techniques, like non-porous particle-packed columns, core-shell and monoliths as chromatographic support matrix, available for achieving fast and efficient chromatographic separation.

Chiral Inversion of Pharmaceutical Drugs - Mini Review.

2-Arylpropionic acid nonsteroidal anti-inflammatory drugs (NSAIDs) provide one of the most demonstrated pharmaceutical examples of chiral inversion. Chiral inversion depends on various factors (. biological-, solvent-, light-, temperature-induced, .

Impact of cyclofructan derivatives as efficient chiral selector in chiral analysis: An overview.

The development of chiral selectors for the separation and analysis of chiral molecules has been an evolving process happening over three decades, since the introduction of the first chiral stationary phase (CSP) in 1938. The main impetus for designing new chiral selectors is to get to most promising one which has a broad chiral recognition property, separation capability for a wide range of chiral analytes, and the cost-effective CSP, which is also a major concern. Today, we have more than 100 commercially available CSPs, and these are prepared by coating or immobilizing the classical chiral selectors on to the chromatographic support, normally, silica gel.

D-Optimal mixture design optimization of an HPLC method for simultaneous determination of commonly used antihistaminic parent molecules and their active metabolites in human serum and urine.

This study describes a specific, precise, sensitive and accurate method for simultaneous determination of hydroxyzine, loratadine, terfenadine, rupatadine and their main active metabolites cetirizine, desloratadine and fexofenadine, in serum and urine using meclizine as an internal standard. Solid-phase extraction method for sample clean-up and preconcentration of analytes was carried out using Phenomenex Strata-X-C and Strata X polymeric cartridges. Chromatographic analysis was performed on a Phenomenex cyano (150 × 4.

Multiple response optimization of a liquid chromatographic method for determination of fluoroquinolone and nitroimidazole antimicrobials in serum and urine.

Objectives: Development and validation of a sensitive, selective and robust SPE assisted HPLC method for the quantification of fluoroquinolones and nitroimidazoles in human serum and urine using design of experiments methodology.

Design And Methods: Design of experiments was employed for method optimization (Box-Behnken design) and robustness testing (Plackett-Burman design). Sample preparation involved a simple solid phase extraction, which offered a satisfactory recovery (≥94%).

Simultaneous enantioseparation and purity determination of chiral switches of amlodipine and atenolol by liquid chromatography.

A novel, selective and robust enantiospecific HPLC method was developed for simultaneous determination of amlodipine and atenolol enantiomers. Box-Behnken design was employed to identify the effect of factors (% ethanol, % diethylamine and flow rate) and their interactions on enantioresolution and analysis time. Chromatography was performed using mobile phase comprising acetonitrile, ethanol and DEA (92:8:0.

Systematic Robustness Testing of a Liquid Chromatographic Method: A Case Study.

Robustness testing of a method plays a crucial role in establishing its reliability. It examines the potential sources of variability in one or more responses of the proposed method. In this study, the robustness testing of a method proposed for simultaneous determination of warfarin and its two process related impurities was evaluated by using two level, fractional factorial design.

An improved HPLC method with the aid of a chemometric protocol: simultaneous analysis of amlodipine and atorvastatin in pharmaceutical formulations.

Statistical experimental design and Derringer's desirability function were applied to develop an improved RP-HPLC method for the simultaneous analysis of amlodipine and atorvastatin in pharmaceutical formulations. Four independent factors were considered: acetonitrile content in the mobile phase; buffer pH; buffer concentration; and flow rate. The preliminary screening step was carried out, according to a 2(4-1) fractional factorial design, to identify the significant factors affecting the analysis time response.