Prep Biochem Biotechnol
January 2025
The study employed batch shake flasks to evaluate the impact of various nitrogen sources, phosphate levels, and sodium acetate (Na-acetate) on the growth and metabolite production. Adding Na-acetate to the medium resulted in significant improvements in critical metabolites. In shake flask experiments, this led to a cell dry weight (CDW) of 1.
View Article and Find Full Text PDFThis study explored the impact of sodium acetate (Na-acetate) impact on lipid, carotenoid, and β-carotene production by the newly isolated strain . Batch and fed-batch bioreactor cultures were employed to optimize growth conditions and product yields. fed with Na-acetate in the yeast medium was evaluated in the batch bioreactor culture.
View Article and Find Full Text PDFThe non-linear complex relationships among the process variables in wastewater and waste gas treatment systems possess a significant challenge for real-time systems modelling. Data driven artificial intelligence (AI) tools are increasingly being adopted to predict the process performance, cost-effective process monitoring, and the control of different waste treatment systems, including those involving resource recovery. This review presents an in-depth analysis of the applications of emerging AI tools in physico-chemical and biological processes for the treatment of air pollutants, water and wastewater, and resource recovery processes.
View Article and Find Full Text PDFTrends Biotechnol
January 2025
The biodegradability of poly (3-hydroxybutyrate) (PHB)-based food packaging material PHB/5GS/0.7MgO, developed by incorporating 5 wt% grapeseed oil (GS) and 0.7 wt% MgO nanoparticles using solution casting route, was investigated in soil and river water environments.
View Article and Find Full Text PDFPoly (3-hydroxybutyrate) (PHB) is undoubtedly a potential substitute for petroleum-based non-biodegradable food packaging materials due to its renewability, high crystallinity, biocompatibility, and biodegradability. Nonetheless, PHB exhibits certain shortcomings, including low flexibility, moderate gas barrier properties, and negligible antimicrobial and antioxidant activities, which limit its direct application in food packaging. Loading essential oils can increase flexibility and induce antimicrobial and antioxidant activities in biopolymers but at the cost of reduced tensile strength.
View Article and Find Full Text PDFBioprocess Biosyst Eng
January 2024
A continuous stirred tank bioreactor (CSTB) with cell recycling combined with ceramic membrane technology and inoculated with Rhodococcus opacus PD630 was employed to treat petroleum refinery wastewater for simultaneous chemical oxygen demand (COD) removal and lipid production from the retentate obtained during wastewater treatment. In the present study, the COD removal efficiency (COD) (%) and lipid concentration (g/L) were predicted using two artificial intelligence models, i.e.
View Article and Find Full Text PDFPoly (3-hydroxybutyrate) (PHB) is a bio-based biodegradable biopolymer with excellent potential to substitute petrochemical-based food packaging materials. Nevertheless, low elongation at break is one of the limiting factors for its commercial-scale application in the packaging field. Microbial contamination and lipid oxidation are the two main causes of food spoilage and pose huge challenges to the food industry.
View Article and Find Full Text PDFPhthalic acid esters (PAEs) are highly toxic compounds and can disrupt the hormonal balance of human, animal, and aquatic organisms. Due to the hazardous nature of such compounds, their removal from constituent wastewater before discharging into the environment is mandatory. This study focused on the biodegradation of dimethyl phthalates (DMP), di-n-butyl phthalates (DBP), and di-n-octyl phthalates (DnOP) by sp.
View Article and Find Full Text PDFEndocrine-disrupting phthalates (EDPs) are widely used as plasticizers for the manufacture of different plastics and polyvinyl chloride by providing flexibility and mechanical strength. On the other hand, they are categorized under priority pollutants list due to their threat to human health and the environment. This study examined biodegradation of a mixture of dimethyl, diethyl, dibutyl, benzyl butyl, di-2-ethylhexyl, and di-n-octyl phthalates using a CSTB (continuous stirred tank bioreactor) operated under batch, fed-batch, continuous, and continuous with biomass recycle operation modes.
View Article and Find Full Text PDFMetallic nanoparticles (NPs) find applications in many different industrial sectors. However, the fate of these NPs in the environment and their potential impact on organisms living in different ecosystems are not fully known. In this work, the individual effect of biogenic and chemically synthesized lead sulfide nanoparticles (PbSNPs) and cadmium sulfide nanoparticles (CdSNPs) on the activity of the oleaginous bacterium Rhodococcus opacus PD630 which belongs to an ecologically important genus Rhodococcus was investigated.
View Article and Find Full Text PDFProduction of value-added products from waste anaerobic digestate is economically and environmentally important for sustainable development of industrial process and products. In this study halophilic microalgae, Chlorella vulgaris 92001, Chlorella vulgaris 50291, Chlorella vulgaris 10241 and Tetraselmis indica, were initially screened for lutein production using synthetic dairy digestate (DD), municipal digestate (MD) and poultry digestate (PD) as no-cost substrates. Screening and optimization of parameters, such as dilution, pH, MgCl, NaCl, NaHCO and inoculum concentration for maximum lutein production were further performed employing statistically designed Plackett-Burman and response surface methodology.
View Article and Find Full Text PDFSelenite (Se) is the most toxic of all the oxyanion forms of selenium. In this study, a feed forward back propagation (BP) based artificial neural network (ANN) model was developed for a fungal pelleted airlift bioreactor (ALR) system treating selenite-laden wastewater. The performance of the bioreactor, i.
View Article and Find Full Text PDFCellulose, starch, chitosan, polylactic acid, and polyhydroxyalkanoates are seen as promising alternatives to conventional plastics in food packaging. However, the application of these biopolymers in the food packaging industry on a commercial scale is limited due to their poor performance and processing characteristics and high production cost. This review aims to provide an insight into the recent advances in research that address these limitations.
View Article and Find Full Text PDFA novel adsorbent based on metal sulfide nanoparticles (MeSNPs) was biologically synthesized from metallic wastewater and examined for azo dyes removal from aqueous solution in batch and continuous systems. The size of the MeSNPs was in the range of 8-10 nm, with an average specific surface area of 120.4 m/g.
View Article and Find Full Text PDFIn this study, paper mill wastewater and hemicellulose hydrolysate were evaluated as low-cost substrates for fungal chitosan production using . Submerged fermentation was first studied using a bioreactor operated under batch, fed-batch and continuous modes with paper mill wastewater as the substrate. Very high removal (91%) of organics as chemical oxygen demand (COD) in the wastewater with 160 mg L chitosan production by was obtained using the bioreactor operated under fed-batch mode for 72 h.
View Article and Find Full Text PDFImproper disposal of huge quantities of fly ash generated by thermal power plants and few other industries contributes to both air and water pollution, and therefore, recent advancements in research are focused toward utilizing this waste material in fabricating useful membranes. This article presents an overview of various methods used to fabricate fly ash-based membranes and critical parameters affecting the same. Fly ash-based membranes also act as the support for fabricating composite membranes and therefore, different means of coating the support membranes are discussed in this paper.
View Article and Find Full Text PDFPhosphate solubilizing bacteria (PSB) that can withstand high cadmium (Cd) stress is a desired combination for bioremediation. This study evaluated the Cd bioremediation potential of four PSB strains isolated from the contaminated soils of a municipal solid waste (MSW) discarding site (Guwahati, India). PSB strains were cultured in Pikovskaya (PVK) media, which led to higher acid phosphatase (ACP) activity and the release of organic acid.
View Article and Find Full Text PDFThis study investigated the potential ofCellulosimicrobium funkeifor degrading dimethyl phthalate (DMP) and diethyl phthalate (DEP). Effect of different initial concentrations of phthalates on their biodegradation and growth ofC. funkeiwas examined using shake flasks and a continuous stirred tank reactor (CSTR).
View Article and Find Full Text PDFMembrane photosynthetic microbial fuel cell (MPMFC) utilizes O, NO and NO as cathodic electron acceptors, enabling simultaneous treatment of nitrogen, CO and organic carbon in the cathode compartment. In this work, development of a novel cathodic process with in situ nitritation via microalgal photosynthesis during the light period is reported for achieving shortcut nitrogen removal (SNR) from ammonium-rich wastewater. Moreover, a tubular low-cost ceramic membrane was used to separate and recycle the microalgal-bacterial biomass to the cathode compartment during the continuous operation.
View Article and Find Full Text PDFAn annular bioreactor (ABR) with wide gap was used for PHB production from Ralstonia eutropha. Hydrodynamic studies demonstrated the uniform distribution of fluid in the ABR due to the Taylor-Couette flow. Thereafter, the ABR was operated at different agitation and sparging rates to study its effect on R.
View Article and Find Full Text PDFA native strain of Bacillus paramycoides isolated from the leachate of coal mine overburden rocks was investigated for its potential to produce selenium nanoparticles (SeNPs) by biogenic reduction of selenite, one of the most toxic forms of selenium. 16S rDNA sequencing was used to identify the bacterial strain (SP3). The SeNPs were characterized using spectroscopic (UV-Vis absorbance, dynamic light scattering, X-ray diffraction, and Raman), surface charge measurement (zeta potential), and ultramicroscopic (FESEM, EDX, FETEM) analyses.
View Article and Find Full Text PDFThe rapid depletion of crude-oil resource which sustains a conventional petroleum refinery together with its environmental impact has led to the search for more sustainable alternatives. In this context, biorefinery serves to fulfil the aim by utilizing waste resources. Hence, this study focused on techno-economic assessment of PHB production at large scale from waste carob pods in a closed-loop biorefinery setup.
View Article and Find Full Text PDFBiological sulfide precipitation by sulfate reducing bacteria (SRB) is an emerging technique for the recovery of heavy metals from metal contaminated wastewater. Advantages of this technique include low capital cost, ability to form highly insoluble salts, and capability to remove and recover heavy metals even at very low concentrations. Therefore, sulfate reduction under anaerobic conditions has become a suitable alternative for the treatment of wastewaters that contain metals.
View Article and Find Full Text PDFA novel closed-loop biorefinery model using carob pods as the feed material was developed for PHB production. The carob pods were delignified, and as the second step, sugars present in the delignified carob pods were extracted using water. Ralstonia eutropha and Bacillus megaterium were cultivated on the carob pod extract and its performance was evaluated using Taguchi experimental design.
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