Case report: Concomitant mutation and rearrangement in non-small cell lung cancer.

In non-small cell lung cancer (NSCLC), two key genetic alterations, epidermal growth factor receptor () mutations and anaplastic lymphoma kinase () rearrangements, are commonly believed to be mutually exclusive. Studies have reported that concurrent co-mutation in non-small cell lung cancer patients is rare, with a prevalence ranging from 0.1% to 1.

CRISPR/Cas-Based Techniques for Live-Cell Imaging and Bioanalysis.

CRISPR/Cas systems have found widespread applications in gene editing due to their high accuracy, high programmability, ease of use, and affordability. Benefiting from the cleavage properties ( or ) of Cas enzymes, the scope of CRISPR/Cas systems has expanded beyond gene editing and they have been utilized in various fields, particularly in live-cell imaging and bioanalysis. In this review, we summarize some fundamental working mechanisms and concepts of the CRISPR/Cas systems, describe the recent advances and design principles of CRISPR/Cas mediated techniques employed in live-cell imaging and bioanalysis, highlight the main applications in the imaging and biosensing of a wide range of molecular targets, and discuss the challenges and prospects of CRISPR/Cas systems in live-cell imaging and biosensing.