Focal adhesion formation of primary human gingival fibroblast on hydrothermally and in-sol-made TiO -coated titanium.

Optimal cell adhesion of the gingival fibroblasts to dental implants is important for maintaining good implant integration. The aim of this study was to discover, if the nanoporous TiO -coating on titanium alloy substrates is able to increase the cell adhesion of the human gingival fibroblasts (HGF). The study consisted of three differently produced titanium groups: hydrothermally produced TiO -coating (HT), novel TiO -coating made in sol (SOL), and noncoated control group.

Epithelial cell attachment and adhesion protein expression on novel in sol TiO coated zirconia and titanium alloy surfaces.

An adequate mucosal attachment is important when it comes to preventing peri-implant inflammation. The aim of this study was to compare epithelial cell adhesion and adhesion protein expression on in sol TiO -coated and non-coated zirconia and titanium alloy surfaces. Fifty-six zirconia and titanium discs were cut, and half of them were coated with bioactive TiO -coating.

Organotypic in vitro block culture model to investigate tissue-implant interface. An experimental study on pig mandible.

In vitro studies of implant-tissue attachment are primarily based on two-dimensional cell culture models, which fail to replicate the three-dimensional native human oral mucosal tissue completely. Thus, the present study aimed to describe a novel tissue culture model using pig mandibular block including alveolar bone and gingival soft tissues to evaluate the tissue attachment to titanium implant provided with hydrothermally induced TiO coating. Tissue attachment on TiO coated and non-coated implants were compared.

Enhancement of Gingival Tissue Adherence of Zirconia Implant Posts: In Vitro Study.

Prevention of bacterial inflammation around dental implants (peri-implantitis) is one of the keys to success of the implantation and can be achieved by securing the gingival tissue-abutment interface preventing penetration of bacteria. Modern dental practice has adopted zirconia abutments in place of titanium, but the adhesion of gingival tissue to zirconia is inferior to titanium. The aim of this study was to assess and improve the adhesion of mucosal tissues to zirconia posts using sol-gel derived TiO coating following dynamic mechanical testing.

Zirconia implants with improved attachment to the gingival tissue.

Background: Gingival tissue attachment is known to be important for long-term prognosis of implants. This in vitro study evaluated the gingival attachment to zirconia implants and zirconia implants modified with sol-gel derived TiO coatings.

Methods: Zirconia endodontic posts (n = 23) were used to function as implants that were inserted into the center of full-thickness porcine gingival explants (n = 31).

TiO Coating and UV Photofunctionalization Enhance Blood Coagulation on Zirconia Surfaces.

This in vitro study was designed to evaluate the effect of sol-gel derived TiO coating on blood coagulation, blood protein adsorption, and platelet response on zirconia surfaces. Square-shaped zirconia (n=96) (10x10x2 mm) was cut, ground, sintered, and finally cleansed ultrasonically in each of acetone and ethanol for 5 minutes. Three experimental groups (n=32) were fabricated: (a) zirconia coated with sol-gel derived TiO, (b) zirconia coated with sol-gel derived TiO and treated with ultraviolet (UV) irradiation for 1 hour, and (c) non-coated zirconia as control.

TiO-Modified Zirconia Surface Improves Epithelial Cell Attachment.

Purpose: Good cell adhesion is an important prerequisite for soft tissue attachment on implant abutment or crown surfaces. The aim of this study was to evaluate the adhesion and proliferation of human epithelial cells on sol-gel-derived TiO-coated and noncoated zirconia.

Materials And Methods: Altogether, 56 zirconia disks (Z-CAD, Metoxit) were fabricated for this study.

Early Biofilm Formation on UV Light Activated Nanoporous TiO Surfaces .

Purpose: To explore early biofilm formation on hydrothermally induced nanoporous TiO surfaces and to examine the effect of UV light activation on the biofilm development.

Materials And Methods: Ti-6Al-4V titanium alloy discs (n = 40) were divided into four groups with different surface treatments: noncoated titanium alloy (NC); UV treated noncoated titanium alloy (UVNC); hydrothermally induced TiO coating (HT); and UV treated titanium alloy with hydrothermally induced TiO coating (UVHT). plaque formation was studied in 10 healthy, nonsmoking adult volunteers.

Effect of ultraviolet light treatment on surface hydrophilicity and human gingival fibroblast response on nanostructured titanium surfaces.

This study was designed to investigate the effect of nanostructured TiO coatings on human gingival fibroblast and to explore the influence of ultraviolet (UV) light on surface wettability and cellular response. Ti-6Al-4V titanium alloy discs ( = 96) were divided into three groups: a sol-gel-derived MetAlive™ (MA) coating; hydrothermal (HT) coating; and a non-coated (NC) group. Forty-eight titanium substrates were further treated with UV light for 15 min.

Ultraviolet photofunctionalization of nanostructured titanium surfaces enhances thrombogenicity and platelet response.

The purpose of this study was to evaluate blood and platelet response to nanostructured TiO coatings and to investigate the effect of Ultraviolet (UV) light treatment on blood clotting ability, platelet activation and protein adhesion. Ti-6Al-4V titanium alloy plates (n = 138) were divided into three groups; a sol-gel derived MetAlive coating (MA); hydrothermal coating (HT); and a non-coated group (NC). Sixty nine titanium substrates were further treated with UV light for 1 h.

Sol-gel derived bioactive coating on zirconia: Effect on flexural strength and cell proliferation.

The purpose of this study was to evaluate the effect of sol-gel derived bioactive coatings on the biaxial flexural strength and fibroblast proliferation of zirconia, aimed to be used as an implant abutment material. Yttrium stabilized zirconia disc-shaped specimens were cut, ground, sintered, and finally cleansed ultrasonically in each of acetone and ethanol for 5 minutes. Three experimental groups (n = 15) were fabricated, zirconia with sol-gel derived titania (TiO ) coating, zirconia with sol-gel derived zirconia (ZrO ) coating, and non-coated zirconia as a control.

Load-bearing capacity of human incisor restored with various fiber-reinforced composite posts.

Objectives: The aim of this study was to evaluate the load-bearing capacity and microstrain of incisors restored with posts of various kinds. Both prefabricated titanium posts and different fiber-reinforced composite posts were tested.

Methods: The crowns of human incisors were cut and post preparation was carried out.

Nanoporous TiO(2) thin film on titanium oral implants for enhanced human soft tissue adhesion: a light and electron microscopy study.

Background: Previous experimental studies have demonstrated direct soft tissue attachment for nanoporous titanium dioxide (TiO(2) ) thin film on implants, while implants without TiO(2) thin film have not shown this capability.

Purpose: The aims were to evaluate and compare TiO(2) surface-modified experimental microimplants with unmodified microimplants with respect to tissue interaction of the human oral mucosa evaluated by light microscopy on ground sections and semithin sections and transmission electron microscopy on ultrathin sections, and to characterize the inflammatory response and the level of the marginal bone resorption.

Materials And Methods: The study was a single-center, randomized, comparative, clinical investigation with intrasubject comparison of implants with and without TiO(2) thin film in 15 patients.

Effect of hydroxyapatite and titania nanostructures on early in vivo bone response.

Purpose: Hydroxyapatite (HA) or titania nanostructures were applied on smooth titanium implant cylinders. The aim was to investigate whether nano-HA may result in enhanced osseointegration compared to nano-titania structures.

Materials And Methods: Surface topography evaluation included detailed characterization of nano-size structures present at the implant surface combined with surface roughness parameters at the micro- and nanometer level of resolution.

Bonding of fibre-reinforced composite post to root canal dentin.

Objective: The aim of this study was to determine bonding properties of two types of fibre-reinforced composite (FRC) posts cemented into root canals of molars. Serrated titanium posts served as reference.

Methods: Prefabricated carbon/graphite FRC posts with cross-linked polymer matrix and individually formed glass FRC posts with interpenetrating polymer network (IPN) polymer matrix were compared.

Exothermal characteristics and release of residual monomers from fiber-reinforced oligomer-modified acrylic bone cement.

The aim of this study is to determine the peak temperature of polymerization, the setting time and the release of residual monomers of a modified acrylic bone cement. Palacos R, a commercial bone cement, is used as the main component. The cement is modified by adding short glass fibers and resorbable oligomer fillers, and an additional cross-linking monomer.

Isocyanato- and methacryloxysilanes promote Bis-GMA adhesion to titanium.

In dentistry, adhesion promotion with 3-methacryloyloxypropyltrimethoxysilane is usually sufficient, but its hydrolytic stability is a continuous concern. The hydrolytic stability of an alternative, 3-isocyanatopropyltriethoxysilane, was compared with that of conventional 3-methacryloyloxypropyltrimethoxysilane. Two silanes, both in 0.

Bonding of composite resin luting cement to fiber-reinforced composite root canal posts.

Purpose: The aim of this study was to compare bonding of composite resin luting cement to a fiber-reinforced composite (FRC) root canal post with either a cross-linked or a semi-interpenetrating polymer network (IPN) polymer matrix.

Materials And Methods: Four different types of prefabricated FRC posts with a cross-linked polymer matrix and two types of FRC posts with a semi-IPN polymer matrix which were individually formed were tested. Serrated titanium posts served as the reference.

Shear bond strength of Bis-GMA resin and methacrylated dendrimer resins on silanized titanium substrate.

Objectives: The study compared the bond strengths of three resins, Bis-GMA and two novel experimental methacrylated polyester dendrimer resins to grit-blasted titanium substrate with three silanes.

Methods: Two commercial dental silanes (ESPE Sil and Monobond-S) and an experimental 0.5 vol% 3-methacryloxypropyltrimethoxysilane were applied to grit-blasted Ti substrates.

Depth of light-initiated polymerization of glass fiber-reinforced composite in a simulated root canal.

Purpose: The possibility of polymerizing glass fiber-reinforced composite (FRC) material into the root canal was preliminarily evaluated by determining the depth of light-initiated polymerization of FRC.

Materials And Methods: The material used was polymer-preimpregnated E-glass fiber reinforcement, which was further impregnated with light-polymerizable dimethacrylate monomer resin. The same resin without fiber reinforcement was used as a control.

Consensus statement on fiber-reinforced polymers: current status, future directions, and how they can be used to enhance dental care.

The Second Scientific Fibre Reinforcement Symposium was held in Nijmegen, The Netherlands, on October 13th, 2001. The participants were invited speakers with extensive scientific and clinical backgrounds in glass fiber and polyethylene fiber research. The symposium reports focused on four areas of fiber reinforcement research: materials development, laboratory testing, clinical systems development, and clinical data.

In vitro evaluation of sol-gel processed spray dried silica gel microspheres as carrier in controlled drug delivery.

The objective of this study was to evaluate sol-gel-derived spray dried silica gel microspheres as carrier material for dexmedetomidine HCl and toremifene citrate. The drug was dissolved in sol-gel processed silica sol before spray drying with Büchi laboratory scale equipment. Microspheres with a low specific surface area were spherical by shape with a smooth surface without pores on the external surface.

Effect of aging time of sol on structure and in vitro calcium phosphate formation of sol-gel-derived titania films.

Titanium and its alloys have been used successfully in the manufacture of orthopedic and dental implants to replace damaged bone tissue. In this study, different sol-gel-derived TiO(2) coatings were produced on titanium substrates using different aging times (5, 10, 24, or 48 h) of the sol before dipping the coatings and varying numbers (one, three, or five) of coating layers. The influence of the aging time of the sol on the structure of the titania coatings with respect to in vitro bioactivity was investigated.

Silica xerogel carrier material for controlled release of toremifene citrate.

Sol-gel processed silica xerogel was used as a carrier material for toremifene citrate in order to develop an implantable controlled release formulation which could be localised to a desired site providing targeted and long-lasting disease control and resulting in a reduced amount of drug needed. Toremifene citrate, an anti-estrogenic compound, was incorporated into silica xerogel matrixes during polycondensation of organic silicate, tetraethyl ortho silicate (TEOS). The effects of drug amount, drying temperature and polyethylene glycol (PEG) on the release rate of toremifene citrate and degradation of the silica xerogel matrixes were investigated.

Silica xerogel as an implantable carrier for controlled drug delivery--evaluation of drug distribution and tissue effects after implantation.

The purpose of the present study was to examine controlled delivery of toremifene citrate from subcutaneously implanted silica xerogel carrier and to evaluate silica xerogel related tissue effects after implantation. Toremifene citrate was incorporated into hydrolyzed silica sol in a room temperature process. Toremifene citrate treated silica xerogel implants were tested both in vitro and in vivo using healthy mice.