A safety study of inactivated Enterovirus 71 vaccine.

Enterovirus 71 (EV71) is one of the major causative agents for hand, foot and mouth disease (HFMD) in childhood. Nowadays, HFMD or EV71 infections have already become an important public health issue throughout the world. Vaccination may be the most effective measure to control the transmission of the virus.

[Preliminary study on RT-PCR-ELISA method for virus titer testing of live attenuated hepatitis A vaccine].

Objective: To evaluate the RT-PCR-ELISA method applied for testing live attenuated hepatitis A vaccine titer.

Methods: A solid phase hybridization-enzyme colorimetric detection method was used for detecting specific nucleic acid. Primer labeled with biotin was used to amplify viral gene fragment, then the product was quickly hybridized with the specific probe covalently coupled on DNA-binding microplate wells.