Publications by authors named "Kang-cheng Pan"
Article Synopsis
- The study aimed to assess the toxic effects of metacavir, an experimental drug for hepatitis B, in rhesus monkeys over a six-month period.
- Observations revealed that while most biological parameters remained stable, significant gastrointestinal, hematological, and liver toxicities were identified, particularly at higher doses.
- The research concluded that metacavir primarily affects the gastrointestinal tract, liver, blood, and kidneys, with a safe dosing level (NOAEL) determined to be 50 mg/kg/day.
Background: Goose parvovirus (GPV) is a Dependovirus associated with latent infection and mortality in geese. Currently, it severely affects geese production worldwide. The objective of this study was to develop a fluorescent quantitative real-time polymerase chain reaction (PCR) (FQ-PCR) assay for fast and accurate quantification of GPV DNA in infected goslings, which can aid in the understanding of the regular distribution pattern and the nosogenesis of GPV in vivo.
View Article and Find Full Text PDFEnterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) is a molecular biological technology that can be used to study microbial community diversity and dynamics. In many reports, investigations of microbial diversity from environmental samples were based on the agarose gel electrophoresis (AGE) patterns of ERIC-PCR amplified products. This is not a sound practice, since bands with identical positions can contain different sequences; thus, this practice could possibly exaggerate the similarities or diversities among samples.
View Article and Find Full Text PDFAim: To develop a simple and convenient method for extracting genomic DNA from intestinal microflora for enterobacterial repetitive intergenic consensus (ERIC)-PCR detection.
Methods: Five methods of extracting bacterial DNA, including Tris-EDTA buffer, chelex-100, ultrapure water, 2% sodium dodecyl sulfate and 10% Triton-100 with and without sonication, were compared with the commercial fecal DNA extraction kit method, which is considered as the gold standard for DNA extraction. The comparison was based on the yield and purity of DNA and the indexes of the structure and property of micro-organisms that were reflected by ERIC-PCR.