[Expression analysis of / double genes in transfected goat mammary epithelial cells].

tPA is a thrombolytic agent widely used in clinical settings. While double gene co-integration into organisms can produce synergistic effects and improved expression levels of the target gene, there are few reports detailing the co-integration of the and genes and an increased expression level of tPA. In order to study this, we obtained monoclonal goat mammary epithelial cell lines with / double gene integration and we analyzed the tPA expression level of single and double gene integration cells.

Affective influences in person-environment fit theory: exploring the role of affect as both cause and outcome of P-E fit.

Person-environment (P-E) fit theory is a general framework that has been used extensively to understand thinking and behavior in organizations. However, recent research has highlighted several important issues that compromise understanding of the P-E fit construct. First, it is widely assumed that affect is only an outcome of P-E fit.

Managing job seekers' organizational image beliefs: the role of media richness and media credibility.

In this article, the authors assessed job seekers' organizational image beliefs before and after they experienced 3 recruitment media. The authors examined whether perceptions of media richness and credibility were related to improvements in the correspondence between job seekers' image beliefs and firms' projected images. Both media richness and credibility perceptions were associated with correspondence between job seekers' image beliefs and firms' projected images.

Use of monoclonal antibodies that recognize p60 for identification of Listeria monocytogenes.

Listeria monocytogenes causes major food-borne outbreaks of disease worldwide. Specific identification of this microorganism is of utmost importance to public health and industry. Listeria species are known to secrete a 60-kDa protein collectively termed p60, which is encoded by the iap (invasion-associated protein) gene and secreted in large quantities into the growth media.

Immunity in the absence of CD28 and CD137 (4-1BB) molecules.

We report the generation and immune regulation of mice that are deficient in CD28 and 4-1BB (CD137) genes. These mice were viable, fertile and did not display any overt abnormalities and had a normal T cell phenotype in thymus and spleen. Proliferative responses to anti-CD3 and ConA were enhanced in 4-1BB-/- but not in either CD28-/- or double mutant mice, while levels of interleukin-2 were decreased in all mutant mice.