Development of a PCR fluorescence sensor utilizing an upconversion FRET system for rapid and ultra-sensitive determination of Escherichia coli.

An upconversion-gold nanoparticle detection system that integrates PCR amplification and fluorescence resonance energy transfer was constructed to enable swift and highly sensitive identification of Escherichia coli. The forward primer used in the PCR amplification is modified with sulfhydryl groups, enabling its connection to gold nanoparticles via Au-S bonds. The complementary strand of the forward primer, which is attached to the upconversion nanomaterials, can hybridize with the free forward primer through base complementary pairing.

Prediction of quality traits in packaged mango by NIR spectroscopy.

Packaging with paper bags is essential for protecting mangoes during growth, but strongly berries near infrared (NIR) spectral signals used for non-invasive analysis of their internal quality. This study focused on eliminating or minimizing the interference of paper bags on the NIR spectra of mangoes and developed innovative solutions to accurately assess mango firmness (FI), dry matter content (DMC), soluble solids content (SSC), and titratable acidity (TA). Specific NIR signals at around 1150-1250 nm and 2100-2400 nm were highlighted, which significantly reduced the precision of NIR predictions for these quality traits.

Exploring the impact of lenticels on the detection of soluble solids content in apples and pears using hyperspectral imaging and one-dimensional convolutional neural networks.

In this work, the effect of lenticels on the predictive performance of apple and pear soluble solids content (SSC) models developed based on hyperspectral imaging (HSI) at 380-1010 nm was investigated for the first time. Variations in the spectral properties of lenticels, pericarp, and combined lenticels and pericarp regions of interest (ROI) were analyzed using two-dimensional correlation spectroscopy method (2D-COS), factor discriminant analysis (FDA) and principal component analysis (PCA). Partial least squares regression (PLSR) was performed to develop calibration models of SSC for each ROI separately.

Discrimination of unsound soybeans using hyperspectral imaging: A deep learning method based on dual-channel feature fusion strategy and attention mechanism.

The application of high-level data fusion in the detection of agricultural products still presents a significant challenge. In this study, dual-channel feature fusion model (DCFFM) with attention mechanism was proposed to optimize the utilization of both one-dimensional spectral data and two-dimensional image data in the hyperspectral images for achieving high-level data fusion. A comparative analysis of support vector machine (SVM), convolutional neural network (CNN) with DCFFM, demonstrated that DCFFM exhibited superior results, achieving the accuracy, precision, recall, specificity, and F1-score of 95.

Exploring the optical response of water status and light propagation in bruised 'Korla' fragrant pear tissues based on low-field nuclear magnetic resonance coupled with Monte Carlo simulation.

In this study, the water distribution and migration of bruised 'Korla' pear flesh during storage, as well as their relationships with optical properties and light propagation, were comprehensively investigated based on the low-field nuclear magnetic resonance (LF-NMR) coupled with Monte Carlo simulations. The immobilized water clearly transferred to free water during pear tissues bruising. The optical absorption coefficient (μ) around 1480 nm was strongly correlated with the LF-NMR T relaxation time of bruised pear tissues by 2D-COS.

An Aptamer Sensor Based on Alendronic Acid-Modified Upconversion Nanoparticles Combined with Magnetic Separation for Rapid and Sensitive Detection of Thiamethoxam.

The widespread use of thiamethoxam has led to pesticide residues that have sparked global concerns regarding ecological and human health risks. A pressing requirement exists for a detection method that is both swift and sensitive. Herein, we introduced an innovative fluorescence biosensor constructed from alendronic acid (ADA)-modified upconversion nanoparticles (UCNPs) linked with magnetic nanoparticles (MNPs) via aptamer recognition for the detection of thiamethoxam.

The effect of hot air treatment on volatile compounds in nectarine fruit and the regulation of glycosidically bound compounds by sugar.

In order to investigate the impact of hot air (HA) treatment on the sugars and volatiles in postharvest nectarine fruit, nectarines were treated with HA at 40 °C for 4 h and stored at 1 °C for 35 days. Changes of sugars, free and glycosidically bound volatiles, β-glucosidase (β-Glu) activity, and the gene expression of UGT (UDP-glucosyltransferase) in nectarine fruit were determined. The results showed that compared with CK, HA treatment delayed the firmness decline of 48.

Integrated omics analysis reveals the interactions among ROS metabolism and malate metabolism associated with chilling tolerance in peach fruit treated with hot air.

Continuous supply of NADPH is necessary for the synthesis of ROS, which can be derived from the decarboxylation of malic acid, providing fuels for RbOHs to sustain ROS production. However, excessive accumulations of ROS lead to significant chilling injury (CI) in peaches during cold storage. Our previous studies indicated that hot air (HA) slows the CI progression in peaches by preventing malate degradation.

Recent Progress in Nanomaterial-Based Fluorescence Assays for the Detection of Food-Borne Pathogens.

Food safety is of great concern, and food-borne bacterial infections and diseases are a major crisis for health. Therefore, it is necessary to develop rapid detection techniques for the prevention and recognition of food safety hazards caused by food-borne pathogens. In recent years, the fluorescence assay has become a widely utilized detection method due to its good signal amplification effect, high detection sensitivity, high stability, and short detection time.

Compact Vital-Sensing Band with Uninterrupted Power Supply for Core Body Temperature and Pulse Rate Monitoring.

Although wearable devices for continuous monitoring of vital signs have undergone significant advancements, their need for frequent recharging precludes continuous operation, potentially leading to adverse outcomes being overlooked. Additionally, the scattered locations of the sensors hamper wearability. Herein, we present a compact vital-sensing band with uninterrupted power supply designed for continuous monitoring of core body temperature (CBT) and pulse rate.

Long-term weight patterns and physical activity in gallstones.

While obesity, rapid weight loss, and sedentary lifestyles contribute to gallstones, the impacts of long-term weight patterns and physical activity on gallstone risk have not been extensively studied at the population level. Using Korea's population-based health database, we created a cohort of 5,062,154 subjects who received over five consecutive biannual health check-ups from 2002 to 2018. Gallstone risk was calculated using hazard ratios (HRs) based on weight patterns (gain, loss, and cycling) and physical activity.

Effects of Changes in Pectin Constitution on Optical Properties and Firmness of Peach Flesh during Storage.

Understanding the fundamental light-sample interaction process is a crucial step toward the development of vibrational spectroscopy to determine fruit texture (i.e., firmness).

Study on color and flavor changes of 4D printed white mushroom gel with microcapsules containing gelatin / β-cyclodextrin induced by microwave heating.

The feasibility of microwave heating to induce color/flavor changes of 4D printed white mushroom gel containing curcumin or γ-dodecalactone (γ-DDL) microcapsules was studied. Using gelatin/ β-cyclodextrin as wall material and soy protein isolate as emulsifier, microcapsules containing curcumin or γ-DDL were prepared by spray drying method. The microcapsules containing curcumin were mixed into white mushroom powder at different mass ratios (0, 0.

Use of optical absorption and scattering properties to monitor the change of chemical characteristics, particle structure and viscoelasticity during apple puree processing.

This work explored the optical absorption (μ) and reduced scattering (μ') properties at 400-1050 nm (Vis-NIR) and 900-1650 nm (SWIR) of apple puree at different heating times to evaluate their changes in composition and structure. The optical absorption and scattering coefficients of μ and μ' decreased with increasing heating time. The specific μ' coefficients at 432 nm, 455 nm, 1183 nm and 1304 nm were related to particle structural changes.

Assessment of optical properties and Monte-Carlo based simulation of light propagation in citrus infected by Penicillium italicum.

This original work investigated the optical properties and Monte-Carlo (MC) based simulation of light propagation in the flavedo of Nanfeng tangerine (NF) and Gannan navel orange (GN) infected by Penicillium italicum. The increase of absorption coefficient (μ) at around 482 nm and the decrease at around 675 nm were both observed in infected NF and GN during storage, indicating the accumulation of carotenoids and loss of chlorophyll. Particularly, the μ in NF varied more intensively than GN, but the limited differences of reduced scattering coefficient (μ') were detected while postharvest infection.

Storage Properties and Shelf-Life Prediction of Fresh-Cut Radishes Treated by Photodynamic Technology.

Fresh-cut radishes are susceptible to quality loss and microbial contamination during storage, resulting in a short shelf life. This study investigated the effects of photodynamic technology (PDT) on fresh-cut radishes stored at 4 °C for 10 d and developed appropriate models to predict the shelf life. Results showed that curcumin-mediated PDT maintained sensory acceptability, color, and firmness, decreased weight loss, and increased ascorbic acid and total phenolics of samples by inactivating polyphenol oxidase and peroxidase, resulting in improved antioxidant capacity and quality.

Visualizing the structural and chemical heterogeneity of fruit and vegetables using advanced imaging techniques: fundamentals, instrumental aspects, applications and future perspectives.

Fruit and vegetables (F&V) are vastly complicated products with highly diverse chemical and structural characteristics. Advanced imaging techniques either combine imaging with spectral information or can provide excellent tissue penetration, and enable the possibility to target, visualize and even qualify the chemical and physical (structural) heterogeneity within F&V. In this review, visible and/or near infrared hyperspectral imaging, Fourier transform infrared microspectroscopic imaging, Raman imaging, X-ray and magnetic resonance imaging to reveal chemical and structural information in a spatial context of F&V at the macro- (entire products), meso- (tissues), and micro- (individual cells) scales are comprehensively summarized.

Introducing high-performance star-shaped bimetallic nanotags into SERS aptasensor: An ultrasensitive and interference-free method for chlorpyrifos detection.

Implementation of cost-effective, reliable, and efficient technologies for the sensitive, rapid, and accurate detection of pesticide residues in agriproducts presents a promising solution to the escalating food safety concerns. Herein, a high-performance surface-enhanced Raman scattering (SERS) aptasensor based on nanotag (AuNS@4-MBN@Ag-aptamer) was introduced for ultrasensitive, reliable, and interference-free detection of chlorpyrifos (CPF). This aptasensor featured star-shaped bimetallic nanotag as the principal Raman signal enhancement material and 4-mercaptobenzonitrile (4-MBN) as "biological-silent"-window reporter (at 2228 cm).

Effect of magnetic field pretreatment on germination characteristics, phenolic biosynthesis, and antioxidant capacity of quinoa.

The development of quinoa-based functional foods with cost-effective methods has gained considerable attention. In this study, the effects of magnetic field pretreatment on the germination characteristics, phenolic synthesis, and antioxidant system of quinoa (Chenopodium quinoa Willd.) were investigated.

Comparing visible and near infrared 'point' spectroscopy and hyperspectral imaging techniques to visualize the variability of apple firmness.

In this work, visible and near-infrared 'point' (Vis-NIR) spectroscopy and hyperspectral imaging (Vis-NIR-HSI) techniques were applied on three different apple cultivars to compare their firmness prediction performances based on a large intra-variability of individual fruit, and develop rapid and simple models to visualize the variability of apple firmness on three apple cultivars. Apples with high degree of intra-variability can strongly affect the prediction model performances. The apple firmness prediction accuracy can be improved based on the large intra-variability samples with the coefficient variation (CV) values over 10%.

Corrigendum to "The Association of Body Mass Index and Waist Circumference with the Risk of Achilles Tendon Problems: A Nationwide Population-Based Longitudinal Cohort Study".

[This corrects the article on p. 488 in vol. 15, PMID: 37274500.

Stilbenes-enriched peanut sprouts alleviated physical fatigue regulating interactions of nutrients-microbiota-metabolites revealed by multi-omics analysis.

In this study, the antifatigue effect and mechanism of peanut sprouts were explored. BALB/c mice divided into three groups (control, dark and UV-C) were respectively supplemented with a normal diet, peanut sprouts (dark germination) added diet and stilbenes-enriched peanut sprouts (UV-C radiated germination) added diet. Results showed that swimming time and levels of blood glucose and antioxidant enzymes significantly increased, while contents of triglyceride and malondialdehyde notably decreased by peanut sprout supplementation.