Fluorophotometric determination of histone with 3,4,5,6-tetrafluoro-2-carboxyphenylfluorone-manganese(II) complex and its characterization.

A simple fluorophotometric method for the determination of histone has been developed. This method involves a fluorescence quenching reaction that results in the formation of a complex of manganese(II), 3,4,5,6-tetrafluoro-2-carboxyphenylfluorone (TFCPF), and histone in a non-ionic surfactant micellar medium. The calibration curve was found to be linear in the range of 0.

[Simple screening method for judging the complex formation between drug and aluminum (III)].

We examined a simple screening method for judging the complex formation between a drug and aluminum(III) on a spot plate. As few drug had color reaction by basing on the binary complex formation of drug-aluminum(III), the ternary complex formation of drug-aluminum(III)-dye was studied this time using 50 kinds of drugs. The dyes used were Chromazurol S and Erythrosin.

Spectrophotometric determination of spermine and related compounds using o-hydroxyhydroquinonephthalein and manganese(II).

A simple and highly sensitive spectrophotometric method for the determination of spermine (Spm) was established based on the ternary complex formation reaction of Spm with o-hydroxyhydroquinonephthalein (QP) as a xanthene dye and manganese(II) as a metal ion in the presence of a dispersion agent. The apparent molar absorptivity at 555 nm and the relative standard deviation of the proposed method were 1.4 x 10(5) dm(3) mol(-1) cm(-1) and 0.

Spectrophotometric determination of uric acid based on fading of o-hydroxyhydroquinonephthalein-palladium(II)-hexadecyltrimethyl-ammonium complex.

A simple and highly sensitive spectrophotometric method for the determination of uric acid (UA) was established based on fading of the o-hydroxyhydroquinonephthalein-palladium(II)-hexadecyltrimethylammonium complex. In the determination of UA, Beer's law is obeyed in the range of 0.01-0.

Spectrophotometric determination of glucosamine and its analogous amino sugars with o-hydroxyhydroquinonephthalein and palladium(II).

A simple and highly sensitive spectrophotometric method for the determination of glucosamine and its analogous amino sugars was established based on fading of the palladium(II)-o-hydroxyhydroquinonephthalein-hexadecyltrimethylammonium complex. In the determination of glucosamine, Beer's law is obeyed in the range of 0.02 - 0.