AEDES ALBOPICTUS IN-HOUSE WINTER BREEDING IN GERMANY-A CASE STUDY.

In February 2024, a resident of Mannheim, Germany, collected aggressively attacking mosquitoes in her home and forwarded them to the "Mueckenatlas" citizen science recording scheme. The specimens turned out to be Aedes albopictus. Upon discussing the situation and consultation on the biology of the Asian tiger mosquito, the submitter found numerous developmental stages in the water of a flowerpot with an orchid in her living room.

Winter activity of Culicoides (Diptera: Ceratopogonidae) inside and outside stables in Germany.

Culicoides biting midge species (Diptera: Ceratopogonidae) of the Obsoletus Group and the Pulicaris Complex are considered the major vectors of bluetongue and Schmallenberg viruses in Europe. Overwintering strategies of these arboviruses are controversially discussed, with the ongoing activity of vector species and a non-disrupted transmission cycle during winter being a plausible explanation. Although data on Culicoides winter activity are relatively scant, a seasonal vector-free period (SVFP), during which adult Culicoides are not or hardly active, is questionable.

Bluetongue Virus Serotype 3 and Schmallenberg Virus in Culicoides Biting Midges, Western Germany, 2023.

In October 2023, bluetongue virus serotype 3 (BTV-3) emerged in Germany, where Schmallenberg virus is enzootic. We detected BTV-3 in 1 pool of Culicoides biting midges collected at the time ruminant infections were reported. Schmallenberg virus was found in many vector pools.

Enhancing arthropod occurrence in wheat cropping systems: the role of non-chemical pest management and nitrogen optimization.

This study analyzes arthropod biomass and abundance to track the changes in arthropod occurrence in relation to pesticide use in three winter wheat cropping systems managed at different intensities (organic, conventional, and hybrid). Arthropod occurrence was surveyed using three collection tools: sweeping nets, eclector traps, and yellow traps. Sampling was conducted over three years from 2020 to 2022 with 588 samples collected.

Large-Scale Cytochrome C Oxidase Subunit I Gene Data Analysis for the Development of a Multiplex Polymerase Chain Reaction Test Capable of Identifying Biting Midge Vector Species and Haplotypes (Diptera: Ceratopogonidae) of the Subgenus Fox, 1955.

The emergence of culicoid-transmitted bluetongue and Schmallenberg viruses in several European countries demonstrated the ability of indigenous biting midge species to transmit pathogens. Entomologic research programs identified members of the Obsoletus Group ( subgenus ) as keyplayers in disease epidemiology in Europe. However, morphological identification of potential vectors is challenging due to the recent discovery of new genetic variants (haplotypes) of sensu stricto (s.

Viral RNA in Mosquitoes (Diptera: Culicidae) Collected between 2019 and 2021 in Germany.

Due to globalisation and climate change, mosquito-borne pathogens are emerging in new areas on all continents, including Europe, which has recently faced outbreaks of dengue, chikungunya and West Nile fever. The present study complements previous investigations to evaluate the circulation of mosquito-borne viruses in Germany, with the aim of identifying potential vector species and risk areas. Mosquitoes collected from 2019 to 2021 and identified to species or species group level were screened for viruses of the families Flaviviridae, Peribunyaviridae and the genus of the family Togaviridae.

Biting Midges (Diptera: Ceratopogonidae) as Vectors of Viruses.

Biting midges of the genus occur almost globally and can regionally and seasonally reach high abundances. Most species are hematophagous, feeding on all groups of vertebrates, including humans. In addition to being nuisance pests, they are able to transmit disease agents, with some viruses causing high morbidity and/or mortality in ruminants, horses and humans.

Avian Plasmodium spp. and Haemoproteus spp. parasites in mosquitoes in Germany.

Background: Although haemosporidian parasites may cause considerable health and economic problems in aviaries, there is limited understanding of the vectors transmitting them. Mosquito-borne Plasmodium species are responsible for the deaths of numerous exotic (= immunologically naïve) birds in zoos every year, while native birds are adapted to the parasites and largely protected by an effective immune response.

Methods: Mosquitoes were collected in bird/animal parks, wetlands and private gardens in various regions of Germany from 2020 to 2022.

Occurrence of putative Culicoides biting midge vectors (Diptera: Ceratopogonidae) inside and outside barns in Germany and factors influencing their activity.

Background: After several years without bluetongue disease, a ruminant illness caused by Culicoides-borne bluetongue virus (BTV), two new autochthonous cases were reported in 2018 in Germany. By contrast, Schmallenberg virus (SBV), another Culicoides-borne virus pathogenic to ruminants, has continuously circulated in Germany since its first emergence in 2011. The disease outbreaks have triggered numerous studies on the biology of the Culicoides vectors, but many ecological details are still obscure.

Demographic and motivational differences between participants in analog and digital citizen science projects for monitoring mosquitoes.

Worldwide, citizen scientists help to map the distribution of native and introduced mosquito species in a variety of programs, contributing to environmental research and management decisions. Participant background, behavior, and engagement may vary depending on the project design, especially between those using digital apps and those using physical samples, which in turn may impact the quality and representativeness of the data collected. During the analysis of the responses to a survey directed at citizen scientists participating in  a digital and an analog program, we found significant differences in the respondents' demographic backgrounds.

Impact of climate change on vector- and rodent-borne infectious diseases.

Background: Endemic and imported vector- and rodent-borne infectious agents can be linked to high morbidity and mortality. Therefore, vector- and rodent-borne human diseases and the effects of climate change are important public health issues.

Methods: For this review, the relevant literature was identified and evaluated according to the thematic aspects and supplemented with an analysis of surveillance data for Germany.

Impact of larvae of the Asian tiger mosquito Aedes albopictus on larvae of the Culex pipiens complex from Germany in laboratory co-breeding studies.

Since the first detection of the Asian tiger mosquito Aedes albopictus (Diptera: Culicidae) eggs in Germany in 2007, several populations of this species have established in Germany. Although colloquially Ae. albopictus is called an 'invasive species', it is not considered 'invasive' and therefore to be controlled according to the European Union (EU) Environmental and Nature Protection Act since evidence of displacement of native species is missing.

Vector Competence of German (Kirby, 1837) for West Nile Virus Lineages 1 and 2.

West Nile virus (WNV) is a zoonotic flavivirus transmitted by mosquitoes as a biological vector. Because of its biting behavior, the widespread snow-melt mosquito could be a potential bridge vector for WNV to humans and nonhuman mammals. However, little is known on its role in transmission of WNV.

Molecular Characterization of Theobald, 1903 (Diptera: Culicidae) Populations from Europe.

Theobald, 1903, considered a competent vector of parasites, is a mosquito species widely distributed in the Mediterranean area but rare in Northern and Central Europe. The disappearance of its suitable breeding sites in Italy is having a detrimental effect on the occurrence of this species once common along the Southern coasts and on the islands. Recently, molecular investigations have renewed interest in this species, highlighting a genetic heterogeneity among European populations.

The microbial RNA metagenome of Aedes albopictus (Diptera: Culicidae) from Germany.

Aedes albopictus is a highly invasive mosquito species that has become widespread across the globe. In addition, it is an efficient vector of numerous pathogens of medical and veterinary importance, including dengue, chikungunya and Zika viruses. Among others, the vector potential of mosquitoes is influenced by their microbiome.

How media presence triggers participation in citizen science-The case of the mosquito monitoring project 'Mückenatlas'.

Since 2012, the citizen science project 'Mückenatlas' has been supplementing the German mosquito monitoring programme with over 28,000 submissions of physical insect samples. As the factors triggering people to catch mosquitoes for science are still unknown, we analysed the influence of mass media reports on mosquito submission numbers. Based on a theoretical framework of how mass media affect citizen responsiveness, we identified five possible influencing factors related to citizen science: (i) project awareness and knowledge, (ii) attention (economy), (iii) individual characteristics of citizen scientists and targeted communication, (iv) spatial differences and varying affectedness, and (v) media landscape.

First Evidence of West Nile Virus Overwintering in Mosquitoes in Germany.

Mosquitoes collected from mid-December 2020 to early March 2021 from hibernacula in northeastern Germany, a region of West Nile virus (WNV) activity since 2018, were examined for WNV-RNA. Among the 6101 mosquitoes tested in 722 pools of up to 12 specimens, one pool of 10 complex mosquitoes collected in early March 2021 in the cellar of a medieval castle in Rosslau, federal state of Saxony-Anhalt, tested positive. Subsequent mosquito DNA analysis produced biotype .

The invasive Korean bush mosquito Aedes koreicus (Diptera: Culicidae) in Germany as of 2020.

Background: The Korean bush mosquito Aedes koreicus was recently reported to have established a population in western Germany (Wiesbaden) in 2016. The species is difficult to distinguish morphologically from its close relative, the invasive Japanese bush mosquito Ae. japonicus, which is already widely distributed in many parts of Germany, including the area colonised by Ae.

Chromosomal and Molecular Diversity in the Group (Diptera: Simuliidae) in the Western Tian Shan Range of Central Asia.

By any measure, such as abundance, species diversity or geographic range, the species group is one of the most successful Palearctic taxa of black flies. To explore potential diversity in this group in the Tian Shan range of Central Asia, we focused on Kyrgyzstan, in which three nominal morphospecies have been recorded. Among our samples, we morphologically identified Rubtsov and a second possible species tentatively identified as Rubtsov.

Field studies on breeding sites of Culicoides LATREILLE (Diptera: Ceratopogonidae) in agriculturally used and natural habitats.

Culicoides are vectors of pathogens mainly of veterinary importance. To establish targeted vector control measures, it is paramount to comprehend the ecological factors determining their distribution. Therefore, we used emergence traps to sample eight biotopes and assess their potential as breeding sites.