Orangutan herpesvirus.

A male orangutan suffered from ulcers at the buccal mucosa. We obtained swab fluid from the base of both vesicles and ulcers and collected blood for further separation into serum, plasma and peripheral blood mononuclear cells (PBMC) for detection of antibody to herpesvirus by serology and herpesvirus DNA by polymerase chain reaction (PCR) using consensus degenerate primers. Serology was positive for human EBV IgG but negative for Epstein-Barr virus (EBV) immunoglobulin (IgM), as well as for both human cytomegalovirus and herpes simplex virus IgG and IgM.

Varicella infection in a pediatric AIDS patient presenting as umbilicated papules.

An 8-year-old girl with acquired immunodeficiency syndrome presented with fever and alteration of consciousness. She had a history of persistent cryptococcal meningitis. She developed multiple discrete umbilicated papules that resembled cutaneous cryptococcosis on the second day of admission.

Detection and differentiation of human herpesviruses 1-5 by consensus primer PCR and RFLP.

Eight human viruses of the Herpesviridae family represent a significant public health problem world-wide. Detection and typing of five of the human herpesviruses (HSV-1, HSV-2, VZV, EBV, and CMV) was performed by applying a consensus primer polymerase chain reaction (PCR). The amplified PCR products from the five human herpesviruses were typed based on their restriction enzyme digestion polymorphism with Hinf I and Alu I.

Serological evidence of herpesvirus infection in gibbons.

Background: Herpesviruses are not only infectious agents of worldwide distribution in humans, but have also been demonstrated in various non-human primates as well. Seventy-eight gibbons were subjected to serological tests by ELISA for herpes simplex virus type 1 (HSV-1), herpes simplex virus type 2 (HSV-2), Epstein-Barr virus (EBV) and cytomegalovirus (CMV).

Results: The prevalence of IgG antibodies against HSV-1, HSV-2, EBV and CMV was 28.