Simple, efficient and economical methods for isolation and estimation of novel isoflavone using RP-HPLC.

The study was undertaken to develop a simplified procedure for the isolation of bioactive isoflavone from , using a direct method of isolation, avoiding the use of chromatographic techniques. The compound was isolated by commercially viable procedure. The extraction of powdered drug (500 g) was done with petroleum ether (60-80) using a Soxhlet apparatus (24 h run).