Differential structure and function of phosphorus-mineralizing microbial communities in organic and upper mineral soil horizons across a temperate rainforest chronosequence.

Microbial community structure and function were assessed in the organic and upper mineral soil across a ~4000-year dune-based chronosequence at Big Bay, New Zealand, where total P declined and the proportional contribution of organic soil in the profile increased with time. We hypothesized that the organic and mineral soils would show divergent community evolution over time with a greater dependency on the functionality of phosphatase genes in the organic soil layer as it developed. The structure of bacterial, fungal, and phosphatase-harbouring communities was examined in both horizons across 3 dunes using amplicon sequencing, network analysis, and qPCR.

Identification of degrader bacteria and fungi enriched in rhizosphere soil from a toluene phytoremediation site using DNA stable isotope probing.

Improved knowledge of the ecology of contaminant-degrading organisms is paramount for effective assessment and remediation of aromatic hydrocarbon-impacted sites. DNA stable isotope probing was used herein to identify autochthonous degraders in rhizosphere soil from a hybrid poplar phytoremediation system incubated under semi-field-simulated conditions. High-throughput sequencing of bacterial 16S and fungal internal transcribed spacer (ITS) genes in metagenomic samples separated according to nucleic acid buoyant density was used to identify putative toluene degraders.

Spatial variability of microbial communities in a fractured sedimentary rock matrix impacted by a mixed organics plume.

Dissolved phase contaminants, transported by diffusion into the low permeability matrix of fractured sedimentary rock, pose a challenge to groundwater cleanup efforts because this stored mass may persist even when the upgradient source zone is removed. In this context, if contaminant biodegradation takes place within the low permeability matrix, plume persistence may be substantially reduced. Therefore, it is important to characterize microbial communities within the low permeability, rock matrix pores, instead of only from groundwater samples, which represent biomass from fast flowing fractures.