Publications by authors named "Kamila Sadowska"

For years, gold nanoparticles (AuNPs) have been widely used in medicine and industry. Although various experimental procedures have been reported for their preparation and manipulation, none of them is optimal for all purposes. In this work, we engineered the N-terminus of the pIII minor coat protein of bacteriophage (phage) M13 to expose a novel HLYLNTASTHLG peptide that effectively and specifically binds gold.

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A simple, cost-effective, one-pot method was proposed to introduce bis-phosphonic groups onto alginic acid and carboxymethyl cellulose (CMC). New derivatives were characterized by means of nuclear magnetic resonance, X-ray photoelectron, and attenuated total reflectance Fourier transform infrared spectroscopy. These analyses confirmed the successful transformation of carboxylic groups present in alginic acid and CMC into bis-phosphonic groups.

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The influence of different solvents, including aqueous and nonaqueous types, on the physicochemical properties of VO nanostructures was thoroughly investigated. Various characterization techniques, such as XRD, XPS, FTIR, Raman spectroscopy, UV-vis DRS, SEM, TEM, and BET, were employed to analyze the obtained materials. Additionally, the adsorption properties of the synthesized VO nanostructures for methylene blue were examined, and kinetic parameters of adsorption were calculated.

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Article Synopsis
  • Antibiotic resistance in bacteria is a major issue in medicine, prompting the search for new treatments, including the use of lytic bacteriophages and metal nanoparticles with antimicrobial properties.
  • Researchers demonstrated that T7 phages combined with silver nanoparticles are more effective at breaking down bacterial biofilms than either treatment alone.
  • The engineered T7 phages, which display a special peptide binding to silver nanoparticles, are effective at low concentrations and do not harm eukaryotic cells, highlighting a promising strategy for tackling bacterial infections safely.
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Introduction: Hybrid nanoflowers are structures consisting of organic (enzymes, proteins, nucleic acids) and inorganic components (mostly metal phosphates) with a flower-like hierarchical structure. Novel hybrid nanoflowers based on bovine serum albumin (BSA) and hydroxyapatite (HA) were obtained and characterized. Study on BSA-HA nanoflowers as potential drug delivery system is reported for the first time.

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The paper describes the development of a novel DNA oligonucleotide-based affinity bioreceptor that binds to lactoferrin, a glycoprotein-type immunomodulator. The research was performed using surface plasmon resonance method to investigate affinity of various types of oligonucleotides to the target protein. The 72 base pair-long 5'[(TAGAGGATCAAA)AAA]TAGAGGATCAAA3' sequence with the highest affinity to lactoferrin was selected for further investigations.

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According to the latest statistics, more than 537 million people around the world struggle with diabetes and its adverse consequences. As well as acute risks of hypo- or hyper- glycemia, long-term vascular complications may occur, including coronary heart disease or stroke, as well as diabetic nephropathy leading to end-stage disease, neuropathy or retinopathy. Therefore, there is an urgent need to improve diabetes management to reduce the risk of complications but also to improve patient's quality life.

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In this paper, we discuss dendrimer usage in enzyme-based electrochemical biosensors, particularly with respect to biomolecule loading on the sensing surface. A novel approach to design bioactive layers with immobilized enzymes for electrochemical biosensors using the surface plasmon resonance (SPR) method in combination with electrochemical impedance spectroscopy was presented. The gold surface was modified with linear linkers (various mercaptoalkanoic acids and aminoalkanethiols) and poly(amidoamine) dendrimers from the first- to fifth-generation.

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The determination of the enzymatic activity requires constant and reproducible measuring conditions, therefore highly stable potentiometric biosensor operating on the basis of coupled enzyme reactions is proposed for arginase activity determination. Glassy carbon electrode was covered with polyazulene ion-to-electron transducing layer, which ensured improved stability of the prepared sensor. The sensor's selectivity was obtained by applying NH-selective membrane on the transducing layer, which was further biofunctionalized with urease via covalent immobilization.

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There is growing interest for bioanalytical tools that might be designed for a specific user, primarily for research purposes. In this perspective, a new, highly stable potentiometric sensor based on glassy carbon/polyazulene/NH-selective membrane was developed and utilized for urease activity determination. Urease-urea interaction studies were carried out and the Michaelis-Menten constant was established for this enzymatic reaction.

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Carbon nanotubes were successfully functionalized for the first time in a free radical phosphonylation reaction. Three synthetic protocols were proposed. Carbon nanotubes and diethylphosphite reacted in the presence of known radical initiator, such as azobisisobutyronitrile, single electron oxidant-Mn(OAc), or under UV radiation.

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A new solid-contact potentiometric ion-selective electrode for the determination of SCN (SCN-ISE) has been described. Synthesized phosphonium derivative of calix[4]arene was used as a charged ionophore. The research included selection of the ion-selective membrane composition, determination of the ISEs metrological parameters and SCN-ISE application for thiocyanate determination in human saliva.

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Ammonium metavanadate, NHVO, plays an important role in the preparation of vanadium oxides and other ammonium compounds, such as NHVO, (NH)VO, and NHVO, which were found to possess interesting electrochemical properties. In this work, a new route for the synthesis of NHVO is proposed by mixing an organic ammonium salt and VO in a suitable solvent. The one-step procedure is carried out at room temperature.

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