Geometry and length control of 3D engineered heart tissues using direct laser writing.

Geometry and mechanical characteristics of the environment surrounding the Engineered Heart Tissues (EHT) affect their structure and function. Here, we employed a 3D tissue culture platform fabricated using two-photon direct laser writing with a high degree of accuracy to control parameters that are relevant to EHT maturation. Using this platform, we first explore the effects of geometry based on two distinct shapes: a rectangular seeding well with two attachment sites, and a stadium-like seeding well with six attachment sites that are placed symmetrically along hemicylindrical membranes.

Effect of torrefaction on fuel properties of biopellets.

The study aimed to determine the effects of torrefaction on the fuel properties of pellets. Therefore, firstly, torrefaction parameters of rose ( Mill.) oil distillation solid waste and red pine sawdust were determined through the torrefaction optimization process in terms of temperature and holding time.

Measurement of the low-frequency charge noise of bacteria.

Bacteria meticulously regulate their intracellular ion concentrations and create ionic concentration gradients across the bacterial membrane. These ionic concentration gradients provide free energy for many cellular processes and are maintained by transmembrane transport. Given the physical dimensions of a bacterium and the stochasticity in transmembrane transport, intracellular ion concentrations and hence the charge state of a bacterium are bound to fluctuate.

Engineering a living cardiac pump on a chip using high-precision fabrication.

Biomimetic on-chip tissue models serve as a powerful tool for studying human physiology and developing therapeutics; however, their modeling power is hindered by our inability to develop highly ordered functional structures in small length scales. Here, we demonstrate how high-precision fabrication can enable scaled-down modeling of organ-level cardiac mechanical function. We use two-photon direct laser writing (TPDLW) to fabricate a nanoscale-resolution metamaterial scaffold with fine-tuned mechanical properties to support the formation and cyclic contraction of a miniaturized, induced pluripotent stem cell-derived ventricular chamber.

Frequency-Dependent Piezoresistive Effect in Top-down Fabricated Gold Nanoresistors.

Piezoresistive strain gauges allow for electronic readout of mechanical deformations with high fidelity. As piezoresistive strain gauges are aggressively being scaled down for applications in nanotechnology, it has become critical to investigate their physical attributes at different limits. Here, we describe an experimental approach for studying the piezoresistive gauge factor of a gold thin-film nanoresistor as a function of frequency.

Direct laser writing for cardiac tissue engineering: a microfluidic heart on a chip with integrated transducers.

We have developed a microfluidic platform for engineering cardiac microtissues in highly-controlled microenvironments. The platform is fabricated using direct laser writing (DLW) lithography and soft lithography, and contains four separate devices. Each individual device houses a cardiac microtissue and is equipped with an integrated strain actuator and a force sensor.

Nanomechanical Measurement of the Brownian Force Noise in a Viscous Liquid.

We study the frequency spectrum of the thermal force giving rise to Brownian motion of a nanomechanical beam resonator in a viscous liquid. In the first set of experiments, we measure the power spectral density (PSD) of the position fluctuations of the resonator around its fundamental mode at its center. Then, we measure the frequency-dependent linear response of the resonator, again at its center, by driving it with a harmonic force that couples well to the fundamental mode.

Aeration requirement and energy consumption of reactor-composting of rose pomace influenced by C/N ratio.

As the composting industry develops rapidly in the world, the compost producers have focused on the efficiency of energy utilization in production without restricting the quality of compost in the forced ventilation systems. Therefore, this experimental study quantified the impacts of initial C/N ratio on aeration requirement and energy consumption due to aeration for reactor composting of rose pomace through kinetics of the process using fifteen 100-l composting reactors. The results of the study showed that initial C/N ratio significantly affected decomposition rate, compost maturity, and dry matter losses and organic matter losses (P < 0.

All-electrical monitoring of bacterial antibiotic susceptibility in a microfluidic device.

The lack of rapid antibiotic susceptibility tests adversely affects the treatment of bacterial infections and contributes to increased prevalence of multidrug-resistant bacteria. Here, we describe an all-electrical approach that allows for ultrasensitive measurement of growth signals from only tens of bacteria in a microfluidic device. Our device is essentially a set of microfluidic channels, each with a nanoconstriction at one end and cross-sectional dimensions close to that of a single bacterium.

Characterization and manipulation of single nanoparticles using a nanopore-based electrokinetic tweezer.

Manipulation and characterization of nanoscale objects through electrokinetic techniques offer numerous advantages compared to the existing optical methods and hold great potential for both fundamental research and practical applications. Here we present a novel electrokinetic tweezer for single nanoparticle manipulation and characterization based on electrokinetic trapping near a low-aspect-ratio nanopore. We find that this nanopore-based electrokinetic tweezer share lots of similarity with optical tweezers and can be modeled as an overdamped harmonic oscillator, with the spring constant of the system being the trap stiffness.

Modeling of road traffic noise and traffic flow measures to reduce noise exposure in Antalya metropolitan municipality.

Background: Road traffic noise influencing directly public health in the modern cities is a growing problem in both developing and developed countries. The objective of this study was to model traffic-induced noise in Antalya province, validate the model with noise emission data, and to run the model for the noise preventive scenarios.

Methods: In this study, modeling of traffic-induced noise was performed using SoundPLAN® software at Gazi Boulevard in the city of Antalya.

The effect of FAS and C/N ratios on co-composting of sewage sludge, dairy manure and tomato stalks.

This study was conducted to determine the effects of C/N ratio and free air space in co-composting of sewage sludge with tomato stalk and dairy manure. Experiments were carried out in 100 L of stainless steel aerobic compost reactors with full automation system and monitored for 32 days. The temperature was controlled according to the Rutgers strategy.

Microfluidic detection of movements of Escherichia coli for rapid antibiotic susceptibility testing.

Various nanomechanical movements of bacteria provide a signature of bacterial viability. Most notably, bacterial movements have been observed to subside rapidly and dramatically when the bacteria are exposed to effective antibiotics. Thus, monitoring bacterial movements, if performed with high fidelity, could offer a path to various clinical microbiological applications, including antibiotic susceptibility tests.

Co-composting of two-phase olive-mill pomace and poultry manure with tomato harvest stalks.

In this study, two-phase olive-mill pomace with poultry manure and chopped tomato harvest stalks were composted at different initial carbon/nitrogen (C/N) ratios with fixed free air space of 35%. Composting experiment was carried out in the 15 aerobic reactors made of stainless steel and was monitored for 28 days. During the composting process, temperature, moisture content, organic matter (OM), pH, electrical conductivity, oxygen and carbon dioxide concentrations, total carbon, total nitrogen, ammonium nitrogen ([Formula: see text]), nitrate nitrogen ([Formula: see text]), and total phosphorus were monitored.

Dynamics of Interstitial Fluid Pressure in Extracellular Matrix Hydrogels in Microfluidic Devices.

In order to understand how interstitial fluid pressure and flow affect cell behavior, many studies use microfluidic approaches to apply externally controlled pressures to the boundary of a cell-containing gel. It is generally assumed that the resulting interstitial pressure distribution quickly reaches a steady-state, but this assumption has not been rigorously tested. Here, we demonstrate experimentally and computationally that the interstitial fluid pressure within an extracellular matrix gel in a microfluidic device can, in some cases, react with a long time delay to external loading.

Co-composting of rose oil processing waste with caged layer manure and straw or sawdust: effects of carbon source and C/N ratio on decomposition.

Rose oil is a specific essential oil that is produced mainly for the cosmetics industry in a few selected locations around the world. Rose oil production is a water distillation process from petals of Rosa damascena Mill. Since the oil content of the rose petals of this variety is between 0.

Non-invasive mapping of interstitial fluid pressure in microscale tissues.

This study describes a non-invasive method for mapping interstitial fluid pressure within hydrogel-based microscale tissues. The method is based on embedding (or forming) a tissue within a silicone (PDMS) microfluidic device, and measuring the extremely slight displacement (<1 μm) of the PDMS optically when the device is pressurized under static and flow conditions. The displacement field under uniform pressure provides a map of the local device stiffness, which can then be used to obtain the non-uniform pressure field under flow conditions.

Monolithic integration of a nanomechanical resonator to an optical microdisk cavity.

We report a Silicon nano-opto-mechanical device in which a nanomechanical doubly-clamped beam resonator is integrated to an optical microdisk cavity. Small flexural oscillations of the beam cause intensity modulations in the circulating optical field in the nearby microdisk cavity. By monitoring the corresponding fluctuations in the cavity transmission via a fiber-taper, one can detect these oscillations with a displacement sensitivity approaching 10 fm·Hz-1/2 at an input power level of 50 μW.

Effect of harvesting hour on some physical and mechanical properties of Rosa damascena Mill.

Background: Mechanization of Rosa damascena flower harvest is limited because of the asynchronous flowering period, the necessity for the harvest operation to take place in the early hour of the morning, and a lack of knowledge regarding the physical-mechanical properties of R. damascena. This study investigated the effect of harvesting hour on some physical-mechanical properties of R.

Sensitive micromechanical displacement detection by scattering evanescent optical waves.

We describe a simple approach to detect small mechanical displacements by scattering evanescent optical waves confined around an optical waveguide. Our experimental setup consists of a microcantilever brought into the proximity of a tapered optical fiber. The scattering of evanescent waves and hence the optical transmission through the tapered fiber is strongly dependent on the separation between the fiber and the microcantilever, allowing for sensitive detection of the small oscillations of the microcantilever.

Long-term spatiotemporal patterns of CH4 and N2O emissions from livestock and poultry production in Turkey.

This study quantified spatiotemporal patterns of CH4 and N2O emissions from livestock and poultry production in Turkey between 1961 and 2007. CH4(enteric) (from enteric fermentation), CH4(manure) (from manure management), and N2O(AWM) (from animal waste management) emissions in Turkey were estimated at 1,164, 216, and 55 Gg in 1961 and decreased to 844, 187, and 39 Gg in 2007, contributing a share of roughly 2% to the global livestock-related CH4 emissions and %1.5 to the global N2O(AWM) emissions, respectively.

Diffraction of evanescent waves and nanomechanical displacement detection.

Sensitive displacement detection has emerged as a significant technological challenge in mechanical resonators with nanometer-scale dimensions. A novel nanomechanical displacement detection scheme based upon the scattering of focused evanescent fields is proposed. The sensitivity of the proposed approach is studied using diffraction theory of evanescent waves.

Efficient and sensitive capacitive readout of nanomechanical resonator arrays.

Here we describe all-electronic broadband motion detection in radio frequency nanomechanical resonators. Our technique relies upon the measurement of small motional capacitance changes using an LC impedance transformation network. We first demonstrate the technique on a single doubly clamped beam resonator with a side gate over a wide range of temperatures from 20 mK to 300 K.