Cell engineering method using fluorogenic oligonucleotide signaling probes and flow cytometry.

Objective: Chromovert® Technology is presented as a new cell engineering technology to detect and purify living cells based on gene expression.

Methods: The technology utilizes fluorogenic oligonucleotide signaling probes and flow cytometry to detect and isolate individual living cells expressing one or more transfected or endogenously-expressed genes.

Results: Results for production of cell lines expressing a diversity of ion channel and membrane proteins are presented, including heteromultimeric epithelial sodium channel (αβγ-ENaC), sodium voltage-gated ion channel 1.