The synthesis of UDP-selective fluorescent probe and its imaging application in living cells.

A perylene-based probe was developed for uridine diphosphate (UDP) sensing and cell imaging. The probe presented about 4-fold fluorescence enhancement in the presence or absence of 100equiv UDP. The selectivity toward UDP over other phosphor-containing anions was observed.

Ammonium-bearing dinuclear copper(II) complex: a highly selective and sensitive colorimetric probe for pyrophosphate.

Two dinuclear copper complexes with and without ammonium moieties were synthesized. The complexes exhibited selective binding affinity to pyrophosphate in aqueous solution. The dinuclear copper complex, with ammonium arms, showed a ca.

Colorimetric and fluorometric assays for acetylcholinesterase and its inhibitors screening based on a fluorescein derivate.

A fluorescein-based sensor was developed for the AChE activity assay and the inhibitor screening. The sensor provided the dual assay methods for the screening of AChE activity in the presence or absence of inhibitor. The colorimetric and fluorometric assays were based on the following processes: (1) owing to the hydrolysis of acetylthiocholine in the presence of AChE, the fluorescein-based probe can rapidly induce 1,4-addition of the hydrolysis product thiocholine to α,β-unsaturated ketone in the compound 1, resulting in strong fluorescence and absorption changes; (2) in the presence of the corresponding inhibitor, the fluorescence enhancement or the absorption change would be inhibited in that the formation of thiocholine was hindered.

Colorimetric and fluorometric detection of neomycin based on conjugated polydiacetylene supramolecules.

Utilizing the colorimetric and fluorogenic changes, a system based on polydiacetylenes (PDAs) is developed for the detection of neomycin. The PDA supramolecules polymerized from the mixed liposome composed of N-(3-hydroxyphenyl)pentacosa-10,12-diynamide (PCDA-AP) and pentacosa-10,12-diynoic acid (PCDA) at an optimized ratio of 1:9 display a unique colorimetric change (blue to red) and fluorescent enhancement in the presence of neomycin. The detection limit for neomycin is estimated to be 2.

Colorimetric and fluorometric assays based on conjugated polydiacetylene supramolecules for screening acetylcholinesterase and its inhibitors.

Polydiacetylene supramolecules (PDAs) are unique sensing materials. Upon environmental stimulation, blue PDAs can undergo a colorimetric transition from blue to red accompanied by fluorescence enhancement. In this paper, we report a new PDA system polymerized from a mixed liposome comprising 2-(2-(2-hydroxyethoxy)ethoxy)ethyl pentacosa-10,12-diynoate and pentacosa-10,12-diynoic acid at a 3:7 ratio.

Synthesis and antioxidant activity of substituted-1,3,2-diazaphosphole 1-oxides.

Synthesis of 1-substituted-1,3,2-diazaphosphole 1-oxides (3a-l) were accomplished via a two-step process. It involves the preparation of diazaphospholo 1-oxide monochloride intermediate (2) and its subsequent reaction with phenols/amino acid esters in dry THF in the presence of triethylamine at 40-45°C. The structures of newly synthesized compounds were characterized by spectral and elemental analysis.