Breaking barriers: Innovative approaches for skin delivery of RNA therapeutics.

RNA therapeutics represent a rapidly expanding platform with game-changing prospects in personalized medicine. The disruptive potential of this technology will overhaul the standard of care with reference to both primary and specialty care. To date, RNA therapeutics have mostly been delivered parenterally via injection, but topical administration followed by intradermal or transdermal delivery represents an attractive method that is convenient to patients and minimally invasive.

Fluorescence-activated cell sorting and directed evolution of α-N-acetylgalactosaminidases using a quenched activity-based probe (qABP).

An α-N-acetylgalactosamine-containing quenched activity-based probe (qABP) was designed and successfully synthesized, and it was subsequently used in directed enzyme evolution experiments aided by fluorescence-activated cell sorting (FACS) for the discovery of an α-N-acetylgalactosaminidase variant with improved catalytic activity.

Direct visual detection of Salmonella genomic DNA using gold nanoparticles.

Gold nanoparticles (AuNPs) are well recognized tools for visual DNA detection. Most reports on their use have however been restricted to synthetic or PCR amplified DNA sequences. Herein, we describe a visual DNA detection method that can detect unamplified genomic DNA sequences specifically, using simple reagents and AuNPs, without the need for PCR.