Isolation, characterization and identification of pesticide degrading bacteria from contaminated soil for bioremediation.

In this study, malathion and chlorpyrifos degrading bacteria were isolated from agricultural soil samples taken from the Himachal region in India. A total of 52 organisms were isolated which were further screened for their efficiency for chlorpyrifos and malathion degradation. Screening was done by checking the growth on Nutrient Agar, Mineral Salt Medium and MacConkey agar plates containing chlorpyrifos and malathion; 37 isolates showed growth in these.

Purification of high molecular weight thermotolerant esterase from sp. and its characterization.

In the present study, an extracellular esterase from sp. was purified 24.46 fold using an initial ammonium sulphate precipitation step (optimized concentration of 30-40%), followed by Diethylaminoethyl cellulose (DEAE-cellulose) chromatography and size exclusion Sephadex G-200 column chromatography steps.

Influence of Culture Conditions on the Production of Extracellular Esterase from Bacillus licheniformis and Its Characterization.

Esterases catalyze the hydrolysis of ester bonds in fatty acid esters with short-chain acyl groups. In the present study, thirty-seven bacterial isolates were isolated from soil contaminated with waste cooking oil, dairy waste etc. from Shimla and Solan district of H.

Application of Lipase Purified from Aspergillus fumigatus in the Syntheses of Ethyl Acetate and Ethyl Lactate.

Microbial lipases are used for the synthesis of various short chain esters such as octyl acetate, methyl salicylate, ethyl acetate and ethyl lactate. In this study, a purified lipase of Aspergillus fumigatus was utilized for the synthesis of two esters i.e.

Synthesis of a PEGylated Dopamine Ester with Enhanced Antibacterial and Antifungal Activity.

Drug-polymer conjugation is a simple and efficient approach to synthesizing new, effective, and potent antimicrobial agents to counter the problem of microbial resistance. In the present study, a PEGylated dopamine ester (PDE) was synthesized using the PEGylation process and synthesis of PDE was confirmed by Fourier-transform infrared spectroscopy, elemental analysis (CHNS-O), and atomic force microscopy techniques. Later, the antimicrobial activity of PDE was assessed against four strains of bacteria (, , , and ; Gram (-)) and two fungi ( and ) by the agar well diffusion method.

Synthesis of Chirally Pure Enantiomers by Lipase.

Pure enantiomers can be produced by chemical and enzymatic methods. Enzymatic methods to produce pure enantiomers are more advantageous. High enantiomeric excess is the advantage of enzymatic method, resulting from the inherent selectivity of the enzyme.

Immobilization of Lipase from Geobacillus sp. and Its Application in Synthesis of Methyl Salicylate.

The present study showed unique properties of an alkaline, thermophilic lipase of Geobacillus sp. which was isolated from soil of hot spring. The study was aimed to investigate the optimum immobilization conditions of lipase onto silica gel matrix (100-200 mesh) by surface adsorption method and its application in the synthesis of methyl salicylate.