One-Pot Glycosylation Strategy Assisted by Ion Mobility-Mass Spectrometry Analysis toward the Synthesis of -Linked Oligosaccharides.

-Glycans are major constituents of several cellular glycoproteins. One-pot strategies for the synthesis of -glycans are crucial for the rapid generation of pure samples to determine their biological functions. Herein, we describe a double one-pot strategy for the synthesis of -glycans assisted by an IM-MS analysis approach for rapid screening of optimized glycosylation reaction conditions.