General features of chromosome substitutions in Triticum aestivum x T. timopheevii hybrids.

Based on a C-banded chromosome analysis of Triticum aestivum x T. timopheevii hybrid lines, we developed a classification of the A(t) and G genome chromosomes that agrees with the standard genetic nomenclature of T. aestivum chromosomes.

Purification and characterization of the immunostimulatory properties of recombinant human interleukin-1 beta.

In this study we have used a new method for human recombinant IL-1 beta (rIL-1 beta) purification and investigated its immunostimulatory biological activity. The IL-1 beta gene was cloned using a novel mRNA preparation from activated human blood monocytes. The purification protocol consists of extraction and two chromatographic steps using the new Soloza cation exchange resin.

Identification of two types of excitatory monosynaptic inputs in frog spinal motoneurones.

Synaptic responses induced in motoneurones by stimulation of dorsal root (DR) and reticular formation (RF) were recorded intracellularly in the isolated frog spinal cord. Argiopine (spider neurotoxin, a selective blocker of glutamate receptors of the non-N-methyl-D-aspartate (non-NMDA) type) in concentrations from 3 x 10(-7) to 1 x 10(-5) M effectively inhibited di- and polysynaptic components of excitatory postsynaptic potentials (EPSPs) induced by DR and RF stimulation. The monosynaptic component of the RF response was inhibited by argiopine, whereas the monosynaptic component of DR response was actually enhanced.

[The heterogeneity of the excitatory synaptic inputs in the spinal motor neurons of the frog Rana ridibunda].

The synaptic responses induced in motoneurones by the stimulations of the dorsal root (DR), single afferent fibres and reticular formation (RF) were intracellularly recorded in the isolated frog spinal cord. It was shown that argiopine (the selective blocker of glutamate receptors of non-NMDA type) in concentrations ranging from 3.10(-7) to 1.

[The neuronal reactions of the area of the tractus solitarius nucleus in the cat to electrical stimulation of the gastric branch of the vagus nerve].

Electrical stimulation of the vagus' gastric branch evoked three types of responses in the solitary tract nucleus of anesthetized cats: phasic discharges, tonic excitation and tonic inhibition. The majority of neurons with phasic and tonic inhibitory types of the responses were found in caudal part of the nucleus. The neurons whose spontaneous activity was increased during repetitive stimulation, were located in the rostral part of the nucleus.

[Physical mapping of the plasmid R205 and identification of a site responsible for increased UV-induced mutability of the bacterial host].

A physical map of the conjugative IncN plasmid R205 (56.1 kb) was constructed. The distribution of cleavage sites for investigated restriction enzymes is asymmetric.

[Properties of the response of frog motoneurons to glutamate application].

The reversal potential (E) of the glutamate response produced by iontophoretically applied glutamate and that of the EPSP's recorded from three synaptic inputs produced by reticular formation (RF), dorsal roots (DS), and microstimulation of presynaptic terminals (PT) at the point of glutamate application were compared at the same motoneuron of the isolated frog spinal cord using current-chop technique. Following Cs injection the glutamate response and EPSP's were reversed. Different points of glutamate application were tested.

[An inhibitor of translation in cellular mRNA containing the 100S structure from Krebs II ascites carcinoma cells infected with encephalomyocarditis virus].

As shown previously, the bulk of cellular mRNA in Krebs II ascite carcinoma cells infected with encephalomyocarditis virus during active virus-specific synthesis is bound to ribosomes within the 100S structure which is inactive in protein synthesis. In order to elucidate the reasons for the translation repression of cellular mRNA within the 100S structure, a fraction of loosely bound proteins which are liberated by treatment of the 100S structure with 0.5 M KCl an which contain sum translation factors, was obtained.

[4,5-S-RNAI in virus-specific polyribosomes from ascitic Krebs 2 carcinoma cells infected with encephalomyocarditis virus].

Polyribosomes of Krebs 2 ascite carcinoma cells non-infected and infected with encephalomyocarditis (EMC) virus contain a heterogeneous population of low molecular weight small RNAs. Analysis of the RNAs by polyacrylamide gel electrophoresis did not reveal any qualitative differences in the small RNA sets within the composition of polyribosomes from virus-infected and non-infected cells. However, the content of one of the small RNAs was markedly elevated in polyribosomes from virus-infected cells.

[Peptidoglycan isolated from Lactobacillus bulgaricus: its effect, mediated by the complement system, on pre-T-cell maturation].

Peptidoglycan from Lactobacillus bulgaricus converts mouse pre-T-cells into theta-positive cells and activates the complement. It has been observed that the effect depends on serum peptidoglycan concentration and the time of the interaction of the activated complement with mouse pre-T-cells. The action of peptidoglycan was replaced by C3a complement component.

[A means of refining the sterilization criterion].

The process efficiency parameter used in estimation of thermal sterilization of nutrient media known as a sterilization criterion is replaced by a new one. A methodical approach to and the results of experimental determination of corrections for the sterilization criterion pattern connected with the operation factors are presented. The following parameters for measuring the criterion were elucidated: contamination of nutrient media, retention time and kinetic characteristics of test-microbe death.

[Properties of informosomes fixed by ultraviolet irradiation].

The conditions for UV irradiation (lambda = 254 nm) of ribonucleoprotein preparations containing informosomes were elaborated which provide for complete fixation of informosomal proteins on RNA. The degree of fixation was controlled by centrifugation in a CsCl density gradient. It was found that complete fixation of informosomes from loach embryos was achieved by irradiation with greater than or equal to 250 quanta per nucleotide.

[Characterization of secondary immune deficiency in patients with chronic glomerulonephritis].

A study was made of the nature of secondary immuno-deficiency, peculiarities of disordered function of regulatory T-lymphocytes (helpers and suppressors), and their interrelationships in different clinical variants of chronic glomerulonephritis (CGN). Altogether 94 CGN patients were examined, of them 34 were with urinary syndrome and 60 with nephrotic syndrome. With relation to a clinical variant of disease the absolute amount of T-lymphocytes was significantly lowered, the amount and function of B-lymphocytes showed a tendency to an increase.

[Involvement of the afterbirth in influenza].

Light- and fluorescence-microscopy examinations of 186 placentas were carried out, with concurrent determination of placental interferon, isolation of influenza virus, and serologic tests of maternal and fetal blood, and amniotic fluid. In 32 of the cases, placentitis caused by serotype A or B of influenza virus was present, characterized by hyperplasia and subsequent destruction of amniotic cells, trophoblast, decidual cells, and vascular endothelium, by the presence of influenza antigens, fuchsinophilic cytoplasmic inclusions in the affected cells, and lymphoid infiltrates, and/or by circulatory disturbances. In addition, an interferon possessing properties of viral, immune, and placental interferon was detected in a number of placentas.

[A new kind of dietetic bread enriched with dietary fiber].

A new sort of dietetic bread has been developed with a 4-fold higher content of food cellulose fibers and a 25% lower fuel value. Such effect was achieved due to the use of qualitatively different sources of food fibers--wheat bran and methyl cellulose (MLI-100). The chemical composition of the new sort of bread has been presented and certain categories of patients have been specified who can use this bread in their diet.