An Environmental-friendly Procedure Based on Deep Eutectic Solvent for Extraction and Determination of Toxic Elements in Fish Species from Different Regions of Iraq.

In this study, an eco-friendly procedure was established by vortex-assisted liquid-phase microextraction based on deep eutectic solvent (VA-LPME-DES) combined with graphite furnace atomic absorption spectroscopy (GFAAS). The performance of this method was demonstrated by the extraction and analysis of lead (Pb), cadmium (Cd), and mercury (Hg) in fish samples. The hydrophobic DES is considered as a green extractant (environmentally friendly and less toxic than common organic solvents) and is a suitable alternative to common toxic organic solvents and is made of l-menthol and ethylene glycol (EG) with a molar ratio of 1:1.