Publications by authors named "Kalima N'Koma Mwange"

Article Synopsis
  • A cDNA clone of the auxin binding protein 1 (ABP1) from Eucommia ulmoides was isolated to study its seasonal expression in relation to plant hormones IAA and ABA using various molecular techniques.
  • The expression of ABP1 varied throughout the cambial stages, being high during active stages and scarce during resting stages, indicating its involvement in the plant's growth cycle.
  • The findings suggest that IAA enhances ABP1 expression and the reactivation of a quiescent cambium, while ABA inhibits it, highlighting ABP1's role in linking auxin response to cambial activity.
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The seasonal change of free abscisic acid (ABA) and indole-3-acetic acid (IAA) and their relationship with the cambial activity in Eucommia ulmoides trees were investigated by ABA and IAA immunolocalization using primary polyclonal and rhodamine-red fluorescing secondary antibodies, ABA and IAA quantification using high performance liquid chromatography (HPLC), and systematic monitoring of vascular cell layers production. ABA and IAA clearly displayed opposite annual distribution patterns. In the active period (AP), both immunolocalization and HPLC detected an abrupt decrease of ABA, reaching its lowest level in the summer.

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Eucommia ulmoides (Eucommiaceae), a traditional Chinese medicinal plant, is often subjected to severe manual peeling of its bark. If the girdled trunk is well protected from desiccation, new bark forms within 1 month. It has been proposed that phytohormones play a key role in this process.

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