Prevalence and molecular analysis of some important viruses in honey bee colonies in Türkiye: the status of multiple infections.

In this study, seven bee viruses of significant importance for bee health in Türkiye were investigated using one-step RT-PCR. For this purpose, larvae from 1183 hives and adult bees from 1196 hives were sampled from 400 apiaries in 40 provinces. The prevalence of viral infections in hives was as follows: acute bee paralysis virus (ABPV), 6.

Oxynoemacheilus fatmae, a new species from the Güzelhisar Stream in the Aegean Sea basin, Türkiye (Teleostei: Nemacheilidae).

Oxynoemacheilus fatmae, a new species, is found in the Güzelhisar Stream in the northern Aegean Sea basin. It is differentiated from all other species of Oxynoemacheilus in the northern Aegean Sea and adjacent basins by having four to eight irregularly shaped narrow black bars on the posterior part of flank, and anterior parts of the flank with a marbled pattern. O.

Oxynoemacheilus marmaraensis, a new species from the Susurluk River, Türkiye (Teleostei: Nemacheilidae).

Oxynoemacheilus marmaraensis, new species, is restricted to the Susurluk River. It is distinguished from all the named species of Oxynoemacheilus in the northwestern Anatolian by the flank with a vermiculate pattern and the presence of a suborbital groove in males, and no axillary lobe at the base of the pelvic fin. It also differs from the closest species, Oxynoemacheilus kentritensis, by having 58 nucleotide substitution sites.

The first detection of betanodavirus reassortant genotype (RGNNV/SJNNV) isolated from gilthead sea bream (Sparus aurata) in the Turkish coastlines: The importance of screening and monitoring studies for identifying the source of the infection.

Viral nervous necrosis (VNN) is now endemic in the Mediterranean basin and the RGNNV genotype betanodavirus has caused frequent epidemics in European sea bass for a long time. Unexpected and increasing VNN epidemics have been reported in gilthead sea bream (GSB) farms in the last few years, from which the RGNNV/SJNNV genotype has been mostly isolated. The aim of this study was to perform a molecular characterization of the betanodavirus isolated from GSB (weighing 90-100 g) in a marine fish farm in the Aegean Sea and also, as an early warning exercise, to investigate the presence/absence of the virus in associated nearby farms (n:20) and in hatcheries (n:3).

Determination of VP2 sequence-based virulence motifs and phylogenetic analysis of domestic Turkish IPNV ısolates.

Infectious pancreatic necrosis (IPN) is a highly contagious disease of young salmonid fish and is one of the most severe economic diseases in aquaculture. In Turkey, an increase in infectious pancreatic necrosis virus (IPNV) outbreaks in freshwater rainbow trout have been reported in recent years. This study aimed to analyze the VP2 gene from recent IPNV isolates from Turkey to determine whether there are epidemiological links between IPNV isolates from rainbow trout (Oncorhynchus mykiss; 62) and sea bass (Dicentrarchus labrax; 1), wild turbot (Scophthalmus maximus; 1) and the environment in order to investigate potential wild and farmed fish interactions.

Inactivated infectious pancreatic necrosis virus (IPNV) vaccine and E.coli-expressed recombinant IPNV-VP2 subunit vaccine afford protection against IPNV challenge in rainbow trout.

Infectious pancreatic necrosis (IPN) is a highly contagious disease causing high mortality in juvenile trouts. Since there is no effective way to treatment against IPNV, early diagnosis and prevention play an important role in combating the disease. The different types of IPNV vaccines (inactive, live, recombinant, DNA, etc) have been produced from local isolates and have been used in developed countries.

Detection of RGNNV genotype betanodavirus in the Black Sea and monitoring studies.

Viral nervous necrosis (VNN), caused by betanodavirus, is a significant viral infection that threatens marine aquaculture. Freshwater and marine fish farms in Turkey are subjected to annual pathogen screenings. In 2016, during the Nervous Necrosis Virus screening program conducted in the Black Sea, betanodavirus was unexpectedly detected using real-time reverse transcription-polymerase chain reaction in apparently healthy sea bass.

The westernmost known population of Paracobitis (Teleostei, Nemacheilidae), with the description of a new species from the Euphrates River in southern Anatolia.

Paracobitis salihae, new species, from the Göksu River in the western upper Euphrates drainage, is distinguished from other Paracobitis species by possessing a truncate caudal-fin, and a dark-brown vermiculate or marbled colour pattern. It is also characterised by 19 variable nucleotide substitutions, three diagnostic nucleotides and 3.6% minimum K2P distance compared to geographically adjacent and related P.

Oxynoemacheilus cilicicus, a new nemacheilid loach from the Göksu River in southern Anatolia (Teleostei: Nemacheilidae).

Oxynoemacheilus cilicicus, new species, is described from the Göksu, Seyhan and Ceyhan rivers in southern Turkey. It is distinguished from other Oxynoemacheilus species in the Eastern Mediterranean Sea basin by possession of an incomplete lateral line, terminating before the vertical of the dorsal-fin origin or slightly behind the dorsal-fin base, a deeply emarginate caudal fin, no suborbital groove in male individuals, and a series of dark-brown bars on the flank not interrupted along the lateral line. Molecular data suggest that Oxynoemacheilus cilicicus is characterised by 22 variable nucleotide substitutions and a minimum K2P distance of 4.

Barbus xanthos, a new barbel from the Southern Aegean basin (Teleostei: Cyprinidae).

Barbus xanthos, a new species, is described from the Eşen, Dalaman, Tersakan and Büyük Menderes rivers in south-western Anatolia. It differs from other Barbus species in the adjacent basins by having 53-60 lateral line scales, a weakly ossified last unbranched dorsal-fin ray (about 33-50%), numerous small irregular-shaped black or dark-brown spots smaller than scales, often forming large, black or dark-brown blotches on back and flank in juveniles and adults, and a straight or slightly convex posterior dorsal-fin margin. B.

Multi-pollutant Monitoring in a Rehabilitated Estuary: Elements and Radionuclides.

The Golden Horn Estuary, Istanbul, Turkey, had been exposed to untreated industrial discharges and municipal wastewater for over fifty years; however, during the last fifteen years pollution has been reduced due to an extensive rehabilitation campaign. Since sediment is the main accumulation matrix of marine ecosystems, surface sediment samples were collected from the estuary and analyzed for concentrations of some elements and radioactivity. The elements of predominant anthropogenic origin such as As, Cu, Pb, Sb, Sn and Zn and Cs were observed in the mid-estuary.

Oxynoemacheilus cemali, a new species of stone loach (Teleostei: Nemacheilidae) from the Çoruh River drainage, Turkey.

Oxynoemacheilus cemali sp. nov. is described from the Çoruh River drainage in the eastern Black Sea basin.

Genetic and molecular evidence that brown trout Salmo trutta belonging to the Danubian lineage are a single biological species.

We tested for reproductive isolation between Salmo trutta abanticus, S.t. labrax, S.

A new species of Petroleuciscus (Teleostei: Cyprinidae) from the Büyük Menderes River, southwestern Anatolia, Turkey.

Petroleuciscus ninae sp. nov. is described from the Büyük Menderes River drainage.

Trophic transfer of Cs in the Manila clam Ruditapes philippinarum.

Bioaccumulation of radiocaesium in many marine organisms occurs through complex trophic transfer mechanisms. The present study addresses the trophic transfer of Cs in the widely distributed marine bivalve, the Manila clam Ruditapes philippinarum, by experimentally determining the assimilation efficiency (AE) and the specific role of food quality or diet on the AE in this marine invertebrate. Pulse-chase feeding experiments were carried out on this clam using the phytoplankton species Tetraselmis chuii, Phaeodactylum tricornutum and Isochrysis galbana.

Diagnosis and surgical correction of congenital bilateral patellar luxation in two dwarf zebu calves.

This case study presents two female dwarf zebu calves with permanent bilateral flexion of the stifle joint secondary to bilateral congenital lateral patellar luxation. The patella could be manually repositioned bilaterally in the first calf (14 days old), but re-luxated immediately with release of manual pressure (luxation grade III). In the second calf (28 days old) both patellae were permanently luxated laterally (grade IV).

Population structure and genetic analysis of narrow-clawed crayfish (Astacus leptodactylus) populations in Turkey.

The genetic differentiation among Turkish populations of the narrow-clawed crayfish was investigated using a partial sequence of cytochrome oxidase subunit I gene (585 bp) of 183 specimens from 17 different crayfish populations. Median joining network and all phylogenetic analyses disclosed a strong haplotype structure with three prominent clades diverged by a range between 20 and 50 mutations and substantial inter-group pairwise sequence divergence (5.19-6.

The usability of tree barks as long term biomonitors of atmospheric radionuclide deposition.

In view of the lower radionuclide activities of moss and lichen, tree barks can be used as biomonitors of radioactive contamination, regardless of the contribution of soil uptake. The present study was conducted to determine the activity concentrations of (137)Cs, (40)K, (232)Th and (238)U in the barks of pine (Pinus nigra) and oak (Quercus petraea) trees collected from the Thrace region in Turkey. By considering the previous studies carried out in the same region, it is noticed that among lichen, moss, oak bark and pine bark, oak bark is the best accumulator of (137)Cs and natural radionuclides.

Oxygen uptake kinetics during cardiopulmonary exercise testing and postoperative complications in patients with lung cancer.

It is accepted that cardiopulmonary exercise testing is one of the most valuable parameters, especially peak oxygen uptake (VO(2)), for the evaluation of risk assessment in lung cancer surgery. It therefore represents an attractive way of identifying a patient at high risk for postoperative complications. However, many patients do not achieve the maximal or predictive level during an incremental exercise testing.

Bronchial sleeve resections for carcinoid tumor in the first decade of life.

Two male patients who were admitted to our department with wheezing, and symptoms of recurrent pneumonia, aged 7 and 10 years, were both diagnosed to have endobronchial masses in the right intermediate bronchus. We employed bronchial sleeve resections with pathological results of carcinoid tumor. Patients were discharged on postoperative days 7 and 8, respectively.

Invasive staging of superior mediastinum in non-small cell lung cancer patients with specific indications.

This prospective study was done between February 2001 and December 2002 on 84 non-small cell lung cancer patients who were apparently operable. We selectively performed mediastinoscopy to 46 patients (54.76%, group 1) with the following indications: clinical T4 tumor, high operative risk, radiologically enlarged mediastinal lymph nodes, clinical T3 tumors with central location, radiologically identified mediastinal lymph nodes of any size with adeno or large cell carcinoma histology.

Prediction of cardiopulmonary morbidity after resection for lung cancer: stair climbing test complications after lung cancer surgery.

Background: The objective of this study was to assess the role of a modified stair climbing test in predicting postoperative cardiopulmonary complications.

Methods: A consecutive series of 150 patients who had undergone pulmonary resection for lung carcinoma formed the prospective database of this study. All patients performed a preoperative modified stair climbing test.

An external support technique for the malacic trachea--treatment of tracheomalacia.

A 76-year-old woman was found to have a large, substernal, intrathoracic goiter. During excision, the trachea was found to be malacic. The trachea was sutured to a 16-mm ringed Gore-Tex (Gore & Associates, Arizona, USA) graft with Vicryl (Ethicon, Johnson & Johnson, USA) sutures.

The diagnostic value of image guided percutaneous fine needle aspiration biopsy in equivocal mediastinal masses.

Background And Aims: The aim of this study was to assess the diagnostic value of image guided percutaneous fine needle aspiration (FNA) biopsy in equivocal mediastinal masses.

Patients: Sixty-six patients with an equivocal mediastinal mass who underwent FNA biopsy between 1993 and 2003 were eligible for final analysis. The cytological and definitive diagnosis of masses were grouped as primary 22 (33%)-30 (46%) and secondary (metastatic) neoplasms 18 (27%)-18 (27%) and nonneoplastic lesions 20 (30%)-18 (27%) respectively.