Publications by authors named "Kako Keiko"
To examine the effects of inflammatory response to plasmid DNA (pDNA) on transgene expression, serum tumor necrosis factor-alpha (TNF-alpha) was measured after intravenous injection of pDNA or calf thymus DNA (CT DNA) in the naked or complexed form with cationic liposomes (lipoplex). pDNA with many CpG motifs induced TNF-alpha production regardless of the forms. No significant TNF-alpha production was detected when CT DNA or methylated pDNA was injected.
View Article and Find Full Text PDFPlasmid DNA (pDNA) expressing mouse interferon (IFN)-beta or IFN-gamma (pCMV-Mu beta and pCMV-Mu gamma, respectively) has been shown to be effective in inhibiting the growth of colon carcinoma CT-26 cells in the liver (Kobayashi et al., Molecular Therapy 2002;6:737-44). The therapeutic effect of such IFN gene transfer could be significantly increased by the sustained expression of IFNs.
View Article and Find Full Text PDFArticle Synopsis
- The study investigates the solution-precipitation method using DMSO in drug discovery, focusing on the solid form of precipitates.
- Findings show that when the precipitates are crystalline and given enough time (20 hours), their solubility matches that of powdered materials.
- An automated birefringence diagnosis system was also developed to enhance the analysis of precipitates in drug discovery.