miR-460b-5p promotes proliferation and differentiation of chicken myoblasts and targets RBM19 gene.

The meat production of broilers is crucial to economic benefits of broiler industries, while the slaughter performance of broilers is directly determined by skeletal muscle development. Hence, the broiler breeding for growth traits shows a great importance. As a kind of small noncoding RNA, microRNA (miRNA) can regulate the expression of multiple genes and perform a wide range of regulation in organisms.

Exploration of Potential Target Genes of miR-24-3p in Chicken Myoblasts by Transcriptome Sequencing Analysis.

Broiler skeletal muscle growth is significantly influenced by miRNAs. Our earlier research demonstrated that miR-24-3p significantly suppressed the proliferation of chicken myoblasts while promoting their differentiation. The purpose of this study is to investigate miR-24-3p potential target genes in chickens.

Transcriptome Sequencing Analysis of circRNA in Skeletal Muscle between Fast- and Slow-Growing Chickens at Embryonic Stages.

Skeletal muscle growth has always been the focus of the broiler industry, and circRNAs play a significant role in this process. We collected leg muscles of slow- and fast-growing Bian chicken embryos in the study at 14 (S14 and F14) and 20 (S20 and F20) days for RNA-seq. Finally, 123 and 121 differentially expressed circRNAs (DECs) were identified in S14 vs.

Prediction of the Effect of Methylation in the Promoter Region of ZP2 Gene on Egg Production in Jinghai Yellow Chickens.

Egg production in chickens is a quantitative trait. The aim of this study was to investigate the effect of promoter methylation of the Zona pellucida 2 (ZP2) gene on egg production. Real-time fluorescence quantification showed that the expression of the ZP2 gene in the ovaries of 300-day-old Jinghai yellow chickens in the high-laying group was significantly higher than that in the low-laying group (p < 0.

miRNA-seq analysis in skeletal muscle of chicken and function exploration of miR-24-3p.

The regulation of skeletal muscle growth and development in chicken is complex. MicroRNAs (miRNAs) have been found to play an important role in the process, and more research is needed to further understand the regulatory mechanism of miRNAs. In this study, leg muscles of Jinghai yellow chickens at 300 d with low body weight (slow-growing group) and high body weight (fast-growing group) were collected for miRNA sequencing (miRNA-seq) and Bioinformatics analysis revealed 12 differentially expressed miRNAs (DEMs) between the two groups.

miRNA-10a-5p Targeting the BCL6 Gene Regulates Proliferation, Differentiation and Apoptosis of Chicken Myoblasts.

Proliferation, differentiation, and apoptosis are three essential stages in cell development, and miRNAs can achieve extensive regulation of cellular developmental processes by repressing the expression of target genes. According to our previous RNA-seq results, miRNA-10a-5p was differentially expressed at different periods in chicken myoblasts, revealing a possible association with muscle development. In this study, we concluded that miRNA-10a-5p inhibited chicken myoblasts' proliferation and differentiation and promoted chicken myoblasts' apoptosis by directly targeting BCL6, a critical transcription factor involved in muscle development and regeneration.

Transcriptome Integration Analysis at Different Embryonic Ages Reveals Key lncRNAs and mRNAs for Chicken Skeletal Muscle.

The growth and development of skeletal muscle at embryonic stages are vital and it directly affects the growth performance of chickens. Long non-coding RNA (lncRNA) plays an important role in this process. In the experiment, we collected the leg muscles of fast- and slow-growing Bian chickens both at 14- and 20-day embryo ages (14E and 20E) for RNA-seq.

Identification of crucial circRNAs in skeletal muscle during chicken embryonic development.

Background: Chicken provides humans with a large amount of animal protein every year, in which skeletal muscle plays a leading role. The embryonic skeletal muscle development determines the number of muscle fibers and will affect the muscle production of chickens. CircRNAs are involved in a variety of important biological processes, including muscle development.

Comparative Analysis of miRNA Expression Profiles in Skeletal Muscle of Bian Chickens at Different Embryonic Ages.

MicroRNAs (miRNAs) are widely involved in the growth and development of skeletal muscle through the negative regulation of target genes. In order to screen out the differentially expressed miRNAs (DEMs) associated with skeletal muscle development of Bian chickens at different embryonic ages, we used the leg muscles of fast-growing and slow-growing Bian chickens at the 14th and 20th embryonic ages (F14, F20, S14 and S20) for RNA-seq. A total of 836 known miRNAs were identified, and 121 novel miRNAs were predicted.

Study on the transcriptome for breast muscle of chickens and the function of key gene RAC2 on fibroblasts proliferation.

Background: Growth performance is significant in broiler production. In the growth process of broilers, gene expression varies at different growth stages. However, limited research has been conducted on the molecular mechanisms of muscle growth and development in yellow-feathered male chickens.

MicroRNA-27b-3p Targets the Myostatin Gene to Regulate Myoblast Proliferation and Is Involved in Myoblast Differentiation.

microRNAs play an important role in the growth and development of chicken embryos, including the regulation of skeletal muscle genesis, myoblast proliferation, differentiation, and apoptosis. Our previous RNA-seq studies showed that microRNA-27b-3p (miR-27b-3p) might play an important role in regulating the proliferation and differentiation of chicken primary myoblasts (CPMs). However, the mechanism of miR-27b-3p regulating the proliferation and differentiation of CPMs is still unclear.

Coordination and Supramolecular Assemblies of Fully Substituted Cyclopentanocucurbit[6]uril with Lanthanide Cations in the Presence of Tetrachlorozincate Anions, and Their Potential Applications.

Coordination and supramolecular assemblies of a fully substituted cyclopentanocucurbit[6]uril (CyPQ[6]) with a series of lanthanide cations (Ln) have been investigated in the presence of tetrachlorozincate anion ([ZnCl]). X-ray single-crystal diffraction analysis has revealed that the interaction of CyPQ[6] and a series of Lncations unexpectedly results in the formation of at least seven different CyPQ[6]-based coordination complex adduct and supramolecular assemblies groups, including with (1) La, Cecations; (2) Pr, Ndcations; (3) Eu, Gd, Tb, Dy with P1̅ or P1 space group, in which CyPQ[6] molecules coordinate alternatively with Lncations and form linear coordination polymers; (4) CyPQ[6] molecules interact alternatively with [Ho(HO)] aqueous complexes and form linear supramolecular chains; CyPQ[6] molecules can assemble two different Ln free porous supramolecular assemblies from CyPQ[6]-Ln(NO)-ZnCl-HCl systems, Ln = Tm, Yb, and Lu; however, no solid crystals were obtained from system containing Ercation. Thus, these differences could lead CyPQ[6] to be useful in not only the isolation of lighter lanthanides from their heavier lanthanides but also special selectivity for different volatile organic compounds.

N-(3,4-Diethoxy-phen-yl)acetamide.

In the title compound, C(12)H(17)NO(3), the conformations of the N-H and C=O bonds are anti to each other. In the crystal structure, N-H⋯O hydrogen-bond inter-actions help to establish the packing.

N'-(Phenyl-sulfon-yl)isonicotinohydrazide monohydrate.

In the title compound, C(12)H(11)N(3)O(3)S·H(2)O, the pyridine ring makes a dihedral angle of 24.78 (14)° with the phenyl ring. Intra-molecular N-H⋯O and inter-molecular O-H⋯O hydrogen bonds are observed and stabilize the packing in the crystal structure.