Silicon dioxide nanoparticles induce anxiety-like behavior in a size-specific manner via the microbiota-gut-brain axis.

The impacts of silicon dioxide nanoparticles (SiO-NPs) on human health have attracted increasing interest due to their widespread utilization in medicine and food additives. However, the size-dependent effects of SiO-NPs on brain health remain sparse. Herein we investigated alterations in behavioral patterns, the gut microbiota, inflammation and oxidative stress of mice after a 12-week exposure to SiO-NPs with either small size (NP-S) or large size (NP-L).

Untargeted metabolomics analysis reveals spatial metabolic heterogeneity in different intestinal segments of type 1 diabetic mice.

Type 1 diabetes (T1D) has been reported to cause systematic metabolic disorders, but metabolic changes in different intestinal segments of T1D remain unclear. In this study, we analyzed metabolic profiles in the jejunum, ileum, cecum and colon of streptozocin-induced T1D and age-matched control (CON) mice by an LC-MS-based metabolomics method. The results show that segment-specific metabolic disorders occurred in the gut of T1D mice.

Exercise Alleviates Behavioral Disorders but Shapes Brain Metabolism of APP/PS1 Mice in a Region- and Exercise-Specific Manner.

Exercise plays a beneficial role in the management of Alzheimer's disease (AD), but its effects on brain metabolism are still far from being understood. Here, we examined behavioral changes of APP/PS1 mice after high-intensity interval training (HIIT) and moderate-intensity continuous training (MICT) and analyzed metabolomics profiles in the hippocampus, cortex, and hypothalamus by using nuclear magnetic resonance spectroscopy to explore potential metabolic mechanisms. The results demonstrate that both HIIT and MICT alleviated anxiety/depressive-like behaviors as well as learning and memory impairments of AD mice.

Optimized integration of metabolomics and lipidomics reveals brain region-specific changes of oxidative stress and neuroinflammation in type 1 diabetic mice with cognitive decline.

Introduction: Type 1 diabetes (T1D) causes cognitive decline and has been associated with brain metabolic disorders, but its potential molecular mechanisms remain unclear.

Objectives: The purpose of this study was to explore the molecular mechanisms underlying T1D-induced cognitive impairment using metabolomics and lipidomics.

Methods: We developed an optimized integration approach of metabolomics and lipidomics for brain tissue based on UPLC-Q-TOF-MS and analyzed a comprehensive characterization of metabolite and lipid profiles in the hippocampus and frontal cortex of T1D male mice with cognitive decline (T1DCD) and age-matched control (CONT) mice.

Identification and Quantification of Chlorogenic Acids from the Root Bark of by UHPLC-Q-Exactive Orbitrap Mass Spectrometry.

The purpose of this study is to identify and quantify the chlorogenic acids (CGAs) from the root bark of , which is conventionally regarded as a tonic in folk Chinese Traditional medicine. The effective methods for identification and quantification analysis of CGAs were developed based on ultra high performance liquid chromatography-Q-exactive orbitrap mass spectrometry (UHPLC-Q-Orbitrap MS) in parallel reaction monitoring (PRM) and selected reaction monitoring (SIM), which showed high sensitivity and resolution for screening and quantifying compounds. The root bark of was extracted under ultrasonication with 70% methanol.

Identification of Metabolites of Aurantio-Obtusin in Rats Using Ultra-High-Performance Liquid Chromatography-Q-Exactive Orbitrap Mass Spectrometry with Parallel Reaction Monitoring.

Aurantio-obtusin (AO) is a major anthraquinone compound isolated from or , which possesses diverse pharmacological effects. Previous studies have shown that it has a good effect on lowering blood lipids and treating various diseases. A few studies have also reported about its metabolites.

Identification of the metabolites of isochlorogenic acid A in rats by UHPLC-Q-Exactive Orbitrap MS.

Content: Isochlorogenic acid A, one of the main components of (Wallich ex Candolle) A. Anderberg (Asteraceae), is a folk medicine used to treat a variety of diseases including fracture and rheumatoid arthritis. Despite its widespread use, the metabolism of isochlorogenic acid A has not been fully studied.

Identification of the Constituents of Percutaneous Absorption from Based on UHPLC-Q-Exactive Orbitrap MS and Microdialysis Technique.

() has been used for treatment of bone fracture by external use. Thus, the percutaneous absorption was crucial to the effect of especially the constituents of percutaneous absorption. However, the constituents were never investigated to date.