Comparative transcriptome profiling reveals the mechanism of increasing lysine and tryptophan content through pyramiding , and genes in maize.

To explore the molecular mechanism behind maize grain quality and use of different gene stacking to improve the nutritional quality of grain, marker-assisted selection (MAS) was used to select three recessive mutant lines containing , along with the double-recessive mutant lines containing , , and . The resulting seeds were taken for transcriptome sequencing analysis 18 days after pollination (DAP). Results: Compared with the recurrent parent genes, in the lysine synthesis pathway, the gene pyramiding lines (, , and ) revealed that the gene encoding aspartate kinase (AK) was up-regulated and promoted lysine synthesis.

Beyond native deoxyribonucleic acid, templating fluorescent nanomaterials for bioanalytical applications: A review.

Due to its unique programmability, nanosized structure, and biocompatible properties, deoxyribonucleic acid (DNA) has attracted increasing attention for the construction of versatile nanostructures and nanomaterials. This review summarizes the recent developments in DNA-templated fluorescent nanomaterials, including DNA-stabilized quantum dots (QDs) and DNA-templated metal nanoclusters (NCs), as well as their applications in bioanalysis. These fluorescent nanomaterials not only have good fluorescence properties but also exhibit excellent performance in DNA recognition, which greatly expands the range of their bioanalytical applications.

Recent progress in copper nanocluster-based fluorescent probing: a review.

Copper nanoclusters (CuNCs) are an attractive alternative to other metal nanoclusters. The synthesis of CuNCs is highly efficient and fast, with low-cost and without any complicated manipulation. Because of their tunable fluorescence and low toxicity, CuNCs have been highly exploited for biochemical sensing.

Detection of micrococcal nuclease for identifying Staphylococcus aureus based on DNA templated fluorescent copper nanoclusters.

Micrococcal nuclease (MNase) is a naturally-secreted nucleic acid degrading enzyme with important role in the spread of the bacteria in an infected host. The content of MNase can be used to estimate the pathogenicity of Staphylococcus aureus (S. aureus).