[The C-terminus of transcription factor TnrA from Bacillus subtilis controls DNA-binding domain activity but is not required for dimerization].

The transcription factor ThrA, which belongs to the MerR transcription regulators, in Bacillus subtilis cells controls genes of nitrogen metabolism under conditions of nitrogen limitation. As all the DNA-binding proteins, it is present as a dimer in cells, but the dimerization site is still unknown. The multiple alignment of TnrA homologs from the other Bacilli allowed to identify the putative dimerization sites.

[The role of AmtB, GlnK and glutamine synthetase in regulation of transcription factor TnrA in Bacillus subtilis].

The nitrogen is a macroelement for all alive cells, from bacteria to animals. Although NH3/NH4+ are highly toxic to animal, they are the preferred source of nitrogen for the most microorganisms and are assimilated by glutamine synthetase in the GOGAT cycle. The nitrogen limitation triggers a number of regulatory processes and activates many genes, providing the utilizing of alternative nitrogen sources.

[Start codon in the serine proteinase gene from Bacillus intermedius].

The translation initiation site in the extracellular serine subtilisin-like proteinase gene from Bacillus intermedius (aprBi) (AN AY754946) secreting at the stationary growth phase was established. The analysis of aprBi open reading frame revealed three putative translation start sites (TTG, GTG, ATG). Using SignalP online freeware program we have determined the functional activity probability of each of them.

[Biosynthesis of the subtilisin-like serine proteinase of Bacillus intermedius under salt stress conditions].

The biosynthesis of the subtilisin-like serine proteinase of Bacillus intermedius 3-19 by the recombinant strain Bacillus subtilis AJ73(pCS9) was found to be enhanced under salt stress conditions (growth in a medium containing 1 M NaCl and 0.25 M sodium citrate). In a recombinant strain of B.

[Biosynthesis of the Bacillus intermedius subtilisin-like serine proteinase by the recombinant Bacillus subtilis strain].

The effect of certain nutrients on the growth and production of the Bacillus intermedius subtilisin-like serine proteinase by the recombinant strain Bacillus subtilis AJ73(pCS9) was studied. Glucose was found to inhibit the synthesis of proteinase in the early (28 h of growth) but not in the late stationary phase (48 h of growth). The inhibitory effect of the other mono- and disaccharides studied was less pronounced.

[The biosynthesis of new secretory high-molecular-weight ribonucleases in Bacillus intermedius and Bacillus subtilis].

The investigation of new secretory ribonucleases, the Bacillus intermedius binase II expressed in the recombinant B. subtilis strain 3922 and the native RNase Bsn of B. subtilis, showed that they are synthesized in the growth retardation phase, when inorganic phosphate is exhausted in the medium.

[Impact of extreme climatic conditions on morphophysiological parameters].

The paper shows that there are uniform changes in the somatotypical characteristics with increases in the age of able-bodied population and in their adaptation to emergency environmental conditions. The amount of an indefinite somatotype increased in all groups (by age, adaptation to the conditions, low- and high lands and high-latitude Antarctic region), major signs of ethnicity and regional traits of the populations remaining in these periods.

[Problems of longevity in Tadzhikistan].

The influence of natural and man-made factors on the longevity of people in Tadzhikistan was studied. The studies indicated that high-altitude hypoxia had an adverse effect on their longevity while premountain and low-mountain areas of the republic were the most beneficial.

[Vascularization of the testes of the white rat in the high altitude conditions of the Pamir and Antarctica].

By means of histometric and morphometric methods vascularization of the white rat testes has been studied at adaptation to living conditions in the Alpian Pamir (at the altitude of 4,000 m) and in the Antarctic Continent (at the altitude of 3,488 m). The leading factor in development of the parenchymatous rearrangements of the testes are certain disturbances of the microcirculatory link in the intraorganic bed. Different degree in manifestation of the morphological signs of the circulatory disturbances are revealed: plethora, interstitial tissue edema, degenerative changes in the spermatogenic epithelium cells and hence decreasing activity of spermatogenesis.