Biochemical evaluation and molecular docking assessment of glucosamines from Neocarya macrophylla fruits against Naja nigricollis venom.

Two new glucosamines, Microphyllose A and B were isolated from the chloroform fraction of Neocarya macrophylla fruit using flash column chromatography. The structures of these compounds were elucidated based on chemical tests and the analysis of their spectral data (IR, 1D- & 2D-NMR). The compounds have demonstrated significant (p < 0.

A serologic relationship among the NFLD, BOW, and Wu red cell antigens.

A survey of sera containing antibodies to multiple low-incidence antigens revealed a variety of patterns of reactions with NFLD+, BOW+, and Wu+ red cell samples. Although NFLD, BOW, and Wu are distinct antigenic determinants (International Society of Blood Transfusion numbers 700.37, 700.

The low-incidence red cell antigen Wra: genetic studies.

Studies of 91 individuals in three families allowed a genetic-linkage analysis of the gene governing the production of the low-incidence red cell antigen Wra and provided evidence that Wra is not a member of the Scianna, Landsteiner-Wiener, Chido/Rodgers, or XK blood group systems, and that the "WR" locus is excluded from autosomal sites or regions 1p34-p22.1, 1p21-q23, 1q32, 2p25, 3q21, 4q28-q32, 6p24-q12, 9q34.1-q34.

Assignment of the Auberger red cell antigen polymorphism to the Lutheran blood group system: genetic justification.

Family studies have provided the final piece of evidence for the assignment of Auberger to the Lutheran blood group system. Lods derived from combined paternal and maternal meioses (zeta = 10.83 at theta = 0.

Distinction of the glycophorin C locus from the Diego, Dombrock and Yt blood group loci.

DNA samples from families informative for the Diego (DI), Dombrock (DO) and Yt (YT) blood group loci were analyzed with a cDNA probe defining a Taq I polymorphism at the glycophorin C locus (GYPC). Recombination between GYPC and DI, DO and YT occurs. Hence GYPC is differentiated from all established blood group system loci.

Linkage between the Colton blood group locus and ASSP11 on chromosome 7.

In an attempt to assign the Colton blood group locus (CO) we have successfully revisited chromosome 7. CO is linked to the argininosuccinate synthetase pseudogene 11 locus (ASSP11) with z = 5.79 at theta = 0.

Genetic evidence that the gene controlling Aub is located on chromosome 19.

DNA from a series of families segregating for Aub was analyzed with a genomic DNA probe which defines a Bg1 I polymorphism for apolipoprotein C II (APOC2). The investigation revealed that the gene for Aub is closely linked to APOC2 (z = 8.43 at theta = 0.

A new, low-incidence red cell antigen (HOFM), associated with depressed C antigen.

A case of mild hemolytic disease of the newborn is presented which was caused by an antibody to a hitherto unknown antigen of low incidence. This antigen, now designated as HOFM (ISBT number 700050) was detected in 6 relatives, and in all of them, it was associated with an unusually weakened expression of C antigen. The serological data indicate that HOFM may be part of the Rh system, but the genetic data, although supportive of this interpretation, are inconclusive.

Evidence for genetic linkage between the KEL and YT blood group loci.

Peak lods (zeta) of 3.48 at an estimated recombination fraction (theta) of 0.28 derived from 63 male and 90 female meioses indicate linkage between the KEL and YT blood group loci.

[Adherence of Candida albicans to acrylic surfaces].

The ability of Candida albicans IFO 1385 to adhere to acrylic and the partial characterization of an adhesive substance, named AS, which was isolated from the yeast, were studied in vitro. The results obtained were as follows: 1. The cells cultured in the synthetic media (YNB) containing 500 mM galactose showed a much greater tendency to adhere than did those cells cultured in the YNB containing 500 mM glucose.

Exclusion of unassigned blood group system loci from the regulator of complement activation gene cluster on chromosome 1q32.

Genetic linkage analyses of the blood group system loci CO, DI, DO, KEL and YT in relation to F13B indicate that these loci are not members of the RCA gene cluster on chromosome 1q32. The data are presented to finalize the exclusion of the DAF carried red cell antigens from all of the 17 established blood group systems.

The Colton blood group locus. A linkage analysis.

Accumulated family information was compiled in an attempt to verify the chromosomal location of the Colton blood group locus (CO). Two-point linkage analysis of CO and 46 other polymorphic loci excludes CO from 1p36 to 1q23, 3q21 to 3q26, 4q13 to 4q28, 6p24 to 6 cen, and 19p13.2 to 19 cen and from linkage groups bounded by ABO and ORM, PI and IGHG, and HP and GOT2.

Blood groups in Newfoundlanders.

Data are presented on 30 high and low incidence antigens and on the distribution of the alleles of 14 blood group systems in a random sample of Newfoundlanders. The distribution of alleles in Newfoundland was compared to founder populations where possible and to the Canadian Caucasian population in general; no significant differences were found. Six rare alleles were observed (IN*a, NFLD, TAR, RH*x, RH*w, RH*V) in a population of 234 individuals.

Analysis for linkage between F13A and three chromosome 6 marker loci: evidence for 6pter:F13A:HLA:GLO1:cen gene order.

The results of the present study provide independent support for F13A:HLA linkage and refine the F13A:HLA and F13A:GLO1 linkage relationships. Analysis of the corresponding recombination fractions for the total paternal F13A:HLA and F13A:GLO1 peak lod scores (z) indicates a locus order of 6pter:F13A:HLA:GLO1:cen. Lod scores between F13A and PLG, a locus recently assigned to chromosome 6, exclude close linkage between these loci.

The chromosome 19 linkage group LDLR, C3, LW, APOC2, LU, SE in man.

The data establish linkage in both sexes for LDLR:LW (zeta = 8.43 at theta = 0.00) and in the male for LDLR:LU (zeta = 3.

The NFLD antigen in Japan.

The low-frequency red cell antigen NFLD was identified in 2 Japanese donors. A family study showed that the antigen is not part of the P1 blood group system. Anti-NFLD was found in serum of several donors (frequency of 0.

The Swann phenotype 700:4,-41; genetic studies.

Serological analysis of the red cells from members of a large French-Canadian kindred proved that the Swa antigen is not part of the P1, Dombrock or Yt blood group systems. A linkage analysis of the SW blood group locus in relation to 27 other loci indicates that SW is not closely linked to ABO, ACP1, ADA, AK1, C3, D2S5, DO, ESD, F13A, FY, GLO1, GPT, HP, IGHG, JK, LU, MYCL, P1, PGP, PGM1, PLG, RH or YT. By inference the study also allows exclusion of Swa from the Landsteiner-Wiener, Radin and Scianna blood group systems and exclusion of SW from the p22.

Degradation of human dentine collagen by an enzyme produced by the yeast Candida albicans.

An extracellular collagenolytic enzyme separated from a culture medium of this pathogenic yeast was found to attack undenatured predentine collagen as seen in scanning electron micrographs. After treatment with the enzyme at pH 4.0, but not by that acidity alone, dentine tubules were less easily distinguished and the collagen fibres were less well-organized.